Anti-APH1a Antibody Picoband™

APH1A antibody

Boster Bio Anti-APH1a Antibody Picoband™ catalog # PB9421. Tested in WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: PB9421
Size: 100 μg/vial
Reactive Species: Human, Mouse
Host: Rabbit
Application: WB

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Product Name

Anti-APH1a Antibody Picoband™

View all APH1A Antibodies

SKU/Catalog Number

PB9421

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-APH1a Antibody Picoband™ catalog # PB9421. Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-APH1a Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9421)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human APH1a, different from the related mouse sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9421 is reactive to APH1A in Human, Mouse

Applications

PB9421 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

29 kDa

Calculated molecular weight

28.996kDa

Background of APH1A

APH1a encodes a component of the gamma secretase complex that cleaves integral membrane proteins such as Notch receptors and beta-amyloid precursor protein. The gamma secretase complex contains this gene product, or the paralogous anterior pharynx defective 1 homolog B (APH1B), along with the presenilin, nicastrin, and presenilin enhancer-2 proteins. The precise function of this seven-transmembrane-domain protein is unknown though it is suspected of facilitating the association of nicastrin and presenilin in the gamma secretase complex as well as interacting with substrates of the gamma secretase complex prior to their proteolytic processing. Polymorphisms in a promoter region of this gene have been associated with an increased risk for developing sporadic Alzheimer's disease. Alternative splicing results in multiple protein-coding and non-protein-coding transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For APH1A (Source: Uniprot.org, NCBI)

Gene Name

APH1A

Full Name

Gamma-secretase subunit APH-1A

Weight

28.996kDa

Superfamily

APH-1 family

Alternative Names

6530402N02Rik; anterior pharynx defective 1 homolog A (C. elegans); APH-1; APH1A; APH-1A; aph-1alpha; CGI-78; gamma-secretase subunit APH-1A; Presenilin-stabilization factor; PSF APH1A 6530402N02Rik, APH-1, APH-1A, CGI-78 aph-1 homolog A, gamma-secretase subunit gamma-secretase subunit APH-1A|APH1A gamma secretase subunit|anterior pharynx defective 1 homolog A|aph-1alpha|presenilin-stabilization factor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on APH1A, check out the APH1A Infographic

APH1A infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for APH1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-APH1a Antibody Picoband™

Question

We are currently using anti-APH1a antibody PB9421 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-10

Answer

The anti-APH1a antibody (PB9421) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-10

Question

Will anti-APH1a antibody PB9421 work on primate WB with glial tumor lung?

Verified Customer

Verified customer

Asked: 2017-09-27

Answer

Our lab technicians have not validated anti-APH1a antibody PB9421 on primate. You can run a BLAST between primate and the immunogen sequence of anti-APH1a antibody PB9421 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate glial tumor lung in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-09-27

Question

I have attached the WB image, lot number and protocol we used for glial tumor lung using anti-APH1a antibody PB9421. Please let me know if you require anything else.

M. Li

Verified customer

Asked: 2016-03-16

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-03-16

Order DetailsPrice
PB9421

100μg

$370
PB9421-10ug

10μg sample (liquid)

$99
PB9421-Biotin

100 μg Biotin conjugated

$570
PB9421-Cy3

100 μg Cy3 conjugated

$570
PB9421-Dylight488

100 μg Dylight488 conjugated

$570
PB9421-Dylight550

100 μg Dylight550 conjugated

$570
PB9421-Dylight594

100 μg Dylight594 conjugated

$570
PB9421-FITC

100 μg FITC conjugated

$570
PB9421-HRP

100 μg HRP conjugated

$570
PB9421-APC

100 μg APC conjugated

$670
PB9421-PE

100 μg PE conjugated

$670
PB9421-carrier-free

Carrier Free

$370

More conjugates/formats

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PB9421
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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