Product Info Summary
SKU: | A06828 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-APLF (Ab-116) Antibody
SKU/Catalog Number
A06828
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-APLF (Ab-116) Antibody (Catalog # A06828). Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-APLF (Ab-116) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06828)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized non-phosphopeptide derived from human APLF around the phosphorylation site of serine 116 (R-N-S(p)-Q-V).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06828 is reactive to Aplf in Human
Applications
A06828 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
54.968kDa
Background of Aplf
Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage.
Saunders W.S., J. Cell Sci. 104:573-582(1993).
Ye Q., J. Biol. Chem. 271:14653-14656(1996).
Ye Q., J. Biol. Chem. 272:14983-14989(1997).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from HT-29 cells, using APLF (Ab-116) antibody A06828.
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Western blot analysis of extracts from COS7 cells (Lane 2), using APLF (Ab-116) antiobdy A06828. The lane on the left is treated with systhesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue using APLF (Ab-116) antibody A06828.
Protein Target Info & Infographic
Gene/Protein Information For Aplf (Source: Uniprot.org, NCBI)
Gene Name
Aplf
Full Name
Aprataxin and PNK-like factor
Weight
54.968kDa
Superfamily
APLF family
Alternative Names
APFL; aprataxin- and PNK-like factor; aprataxin and PNK-like factor; aprataxin and PNKP like factor; Apurinic-apyrimidinic endonuclease APLF; C2orf13FLJ16593; chromosome 2 open reading frame 13; EC 4.2.99.18; MGC47799; PALF; PNK and APTX-like FHA domain-containing protein; PNK and APTX-like FHA protein; Xip1; XRCC1-interacting protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Aplf, check out the Aplf Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Aplf: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-APLF (Ab-116) Antibody (A06828)
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No publications found for A06828
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