Anti-Amyloid-like protein 2 APLP2 Antibody

Product Name

Anti-Amyloid-like protein 2 APLP2 Antibody

View all APLP-2 Antibodies

SKU/Catalog Number

PA1654-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Amyloid-like protein 2 APLP2 Antibody catalog # PA1654-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Amyloid-like protein 2 APLP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1654-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human APLP2, different from the related rat and mouse sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1654-1 is reactive to APLP2 in Human, Mouse, Rat

Applications

PA1654-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

100-110 kDa

Calculated molecular weight

86.956kDa

Background of APLP-2

APLP2 (Amyloid beta (A4) precursor-like protein 2), also known as CDEBP or SPERM MEMBRANE PROTEIN, is a protein that in humans is encoded by the APLP2 gene. The APLP2 gene is mapped on 11q24.3. The human amyloid precursor-like protein APLP2 is a highly conserved homolog of a sequence-specific DNA-binding mouse protein with an important function in the cell cycle. APLP2 along with APLP1 are important modulators of glucose and insulin homeostasis. APLP2 associates with antigen presentation molecules like MHC class I molecules and regulates their surface expression by enhancing endocytosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of APLP2 using anti-APLP2 antibody (PA1654-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APLP2 antigen affinity purified polyclonal antibody (Catalog # PA1654-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APLP2 at approximately 100-110 kDa. The expected band size for APLP2 is at 87 kDa.

Gene/Protein Information For APLP2 (Source: Uniprot.org, NCBI)

Alternative Names

amyloid beta (A4) precursor-like protein 2; amyloid precursor protein homolog HSD-2; Amyloid protein homolog; amyloid-like protein 2; APLP2; APLP-2; APPH; APPHCDEI box-binding protein; APPL2; CDEBP; WALPLP2; YWK-II APLP2 APLP-2, APPH, APPL2, CDEBP amyloid beta precursor like protein 2 amyloid-like protein 2|CDEI box-binding protein|amyloid beta (A4) precursor-like protein 2|amyloid precursor protein homolog HSD-2|testicular tissue protein Li 23

For more info on APLP2, check out the APLP2 Infographic

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In this infographic, you will see the following information for APLP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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