Anti-APLP2 Antibody

Rabbit Polyclonal APLP2 Antibody. Validated in WB and tested in Human, Mouse, Rat.

SKU PA1654-1
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications WB

Overview

Product Name Anti-APLP2 Antibody
See all APLP2 primary antibodies, ELISA kits and proteins
SKU/Catalog Number PA1654-1
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit Polyclonal APLP2 Antibody. Validated in WB and tested in Human, Mouse, Rat. Size: 100μg/vial.
Cite This Product Anti-APLP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1654-1)
Specificity Anti-APLP2 Antibody (PA1654-1) reacts with Human, Mouse, Rat APLP2, in native form and recombinant. Superfamily members of APLP2 are not reactive to PA1654-1.
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Form Lyophilized
Immunogen Anti-APLP2 Antibody (PA1654-1) was raised against A synthetic peptide corresponding to a sequence at the C-terminus of human APLP2(639-656aa EEKVINSKNKVDENMVID), different from the related rat and mouse sequences by one amino acid..
Reactivity Human, Mouse, Rat

Assay Details

Our Boster Quality Guarantee for Anti-APLP2 Antibody covers its use in the following applications.

*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Boster's Compatible Products

The following reagents are used to generate the images below for Anti-APLP2 Antibody (PA1654-1).

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Images And Assay Conditions

/antibody/pa1654 1 1 WB anti aplp2 antibody.jpg

Anti-APLP2 antibody, PA1654-1, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Heart Tissue Lysate
Lane 3: SMMC Cell Lysate

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q06481
Gene Name APLP2
Protein Name Amyloid-like protein 2
Tissue Specificity Expressed in placenta, brain, heart, lung, liver, kidney and endothelial tissues.
Alternative Names Amyloid-like protein 2;APLP-2;APPH;Amyloid protein homolog;CDEI box-binding protein;CDEBP;APLP2;APPL2;Show more
Subcellular Localization Cell membrane ; Single-pass type I membrane protein . Nucleus .
Molecular Weight 86956 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function May play a role in the regulation of hemostasis. The soluble form may have inhibitory properties towards coagulation factors. May interact with cellular G-protein signaling pathways. May bind to the DNA 5'-GTCACATG-3'(CDEI box). Inhibits trypsin, chymotrypsin, plasmin, factor XIA and plasma and glandular kallikrein. Modulates the Cu/Zn nitric oxide-catalyzed autodegradation of GPC1 heparan sulfate side chains in fibroblasts (By similarity). .
Research Areas Alzheimer'S Disease, Neurodegenerative Disease, Neurogenesis, Neurology Process, Neuroscience

*You can search these to find other products in these research areas.
Background APLP2(Amyloid beta(A4) precursor-like protein 2), also known as CDEBP or SPERM MEMBRANE PROTEIN, is a protein that in humans is encoded by the APLP2 gene. The APLP2 gene is mapped on 11q24.3. The human amyloid precursor-like protein APLP2 is a highly conserved homolog of a sequence-specific DNA-binding mouse protein with an important function in the cell cycle. APLP2 along with APLP1 are important modulators of glucose and insulin homeostasis. APLP2 associates with antigen presentation molecules like MHC class I molecules and regulates their surface expression by enhancing endocytosis.

Other Recommended Resources

Here are featured tools and databases that you might find useful.

Publishing with Anti-APLP2 Antibody (PA1654-1)? Please let us know so we can cite the reference in this product datasheet. Email us at support@bosterbio.com.

Order Product (PA1654-1)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Option Price
30ug sample size $99
100ug $240
100ug+Free HRP Secondary BA1054 $240
100ug+Free Biotin Secondary BA1003 $240

USD $240

Ships next business day.

Troubleshooting

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Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.