Anti-ApoB/Apolipoprotein B Rabbit Monoclonal Antibody

APOB antibody

Boster Bio Anti-ApoB/Apolipoprotein B Rabbit Monoclonal Antibody catalog # M00100. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human.

Product Info Summary

SKU: M00100
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IP, WB

Product Name

Anti-ApoB/Apolipoprotein B Rabbit Monoclonal Antibody

View all APOB Antibodies

SKU/Catalog Number

M00100

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-ApoB/Apolipoprotein B Rabbit Monoclonal Antibody catalog # M00100. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ApoB/Apolipoprotein B Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00100)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AOIH-1

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human ApoB

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00100 is reactive to APOB in Human

Applications

M00100 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee

Observed Molecular Weight

21 kDa

Calculated molecular weight

515.605kDa

Background of APOB

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
FC 1:100
IP 1:50

Validation Images & Assay Conditions

Gene/Protein Information For APOB (Source: Uniprot.org, NCBI)

Gene Name

APOB

Full Name

Apolipoprotein B-100

Weight

515.605kDa

Alternative Names

Apo B-100; APOB; ApoB-100; ApoB-48; apolipoprotein B (including Ag(x) antigen); Apolipoprotein B; apolipoprotein B-100; apolipoprotein B48; FLDB; LDLCQ4; mutant Apo B 100 APOB FCHL2, FLDB, LDLCQ4, apoB-100, apoB-48 apolipoprotein B apolipoprotein B-100|apolipoprotein B (including Ag(x) antigen)|apolipoprotein B48

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on APOB, check out the APOB Infographic

APOB infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for APOB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
M00100

100μL

$370
M00100-30ul

30μL sample

$99
M00100-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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