Product Info Summary
SKU: | M00708 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Mouse |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-APOBEC3G Antibody Picoband™ (monoclonal, 6C2)
SKU/Catalog Number
M00708
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-APOBEC3G Antibody Picoband™ (monoclonal, 6C2) catalog # M00708. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-APOBEC3G Antibody Picoband™ (monoclonal, 6C2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00708)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
6C2
Isotype
Mouse IgG1
Immunogen
E.coli-derived human APOBEC3G recombinant protein (Position: E191-N384).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M00708 is reactive to APOBEC3G in Human
Applications
M00708 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
46 kDa
Calculated molecular weight
46.408kDa
Background of APOBEC3G
APOBEC3G (apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G) is a human enzyme encoded by the APOBEC3G gene. This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. The protein encoded by this gene has been found to be a specific inhibitor of human immunodeficiency virus-1 (HIV-1) infectivity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry, 1-3μg/1x106 cells
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of APOBEC3G using anti-APOBEC3G antibody (M00708).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Raji whole cell lysates,
Lane 2: K562 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-APOBEC3G antigen affinity purified monoclonal antibody (Catalog # M00708) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for APOBEC3G at approximately 46KD. The expected band size for APOBEC3G is at 46KD.
Click image to see more details
Figure 2. IHC analysis of APOBEC3G using anti-APOBEC3G antibody (M00708).
APOBEC3G was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-APOBEC3G Antibody (M00708) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of APOBEC3G using anti-APOBEC3G antibody (M00708).
APOBEC3G was detected in paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-APOBEC3G Antibody (M00708) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of APOBEC3G using anti-APOBEC3G antibody (M00708).
APOBEC3G was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-APOBEC3G Antibody (M00708) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 5. Flow Cytometry analysis of THP-1 cells using anti-APOBEC3G antibody (M00708).
Overlay histogram showing THP-1 cells stained with M00708 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-APOBEC3G Antibody (M00708,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 6. IF analysis of APOBEC3G using anti-APOBEC3G antibody (M00708).
APOBEC3G was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-APOBEC3G Antibody (M00708) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For APOBEC3G (Source: Uniprot.org, NCBI)
Gene Name
APOBEC3G
Full Name
DNA dC->dU-editing enzyme APOBEC-3G
Weight
46.408kDa
Superfamily
cytidine and deoxycytidylate deaminase family
Alternative Names
APOBEC-related cytidine deaminase; APOBEC-related protein 9; APOBEC-related protein; apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G; ARCD; ARP-9; bK150C2.7; CEM-15; CEM15ARP9; dJ494G10.1; DNA dC->dU-editing enzyme APOBEC-3G; EC 3.5.4; EC 3.5.4.-; EC 3.5.4.5; FLJ12740DNA dC->dU editing enzyme; MDS019; phorbolin-like protein MDS019 APOBEC3G A3G, ARCD, ARP-9, ARP9, CEM-15, CEM15, MDS019, bK150C2.7, dJ494G10.1 apolipoprotein B mRNA editing enzyme catalytic subunit 3G DNA dC->dU-editing enzyme APOBEC-3G|APOBEC-related cytidine deaminase|APOBEC-related protein 9|DNA dC->dU editing enzyme|apolipoprotein B editing enzyme catalytic polypeptide-like 3G|apolipoprotein B mRNA editing enzyme cytidine deaminase|apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G|apolipoprotein B mRNA-editing enzyme catalytic polypeptide 3G|deoxycytidine deaminase|phorbolin-like protein MDS019
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on APOBEC3G, check out the APOBEC3G Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for APOBEC3G: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-APOBEC3G Antibody Picoband™ (monoclonal, 6C2) (M00708)
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Customer Q&As
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3 Customer Q&As for Anti-APOBEC3G Antibody Picoband™ (monoclonal, 6C2)
Question
I would like to test anti-APOBEC3G antibody (monoclonal, 6C2) M00708 on human t-cell lymphoma for research purposes, then I may be interested in using anti-APOBEC3G antibody (monoclonal, 6C2) M00708 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-01
Answer
The products we sell, including anti-APOBEC3G antibody (monoclonal, 6C2) M00708, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-01
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for t-cell lymphoma using anti-APOBEC3G antibody (monoclonal, 6C2) M00708. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-27
Question
I was wanting to use your anti-APOBEC3G antibody (monoclonal, 6C2) for IHC-P for human t-cell lymphoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human t-cell lymphoma identification?
W. Anderson
Verified customer
Asked: 2017-05-05
Answer
As indicated on the product datasheet, M00708 anti-APOBEC3G antibody (monoclonal, 6C2) has been tested for Flow Cytometry, IHC-P, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human t-cell lymphoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-05-05