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Product Info Summary
| SKU: | M03444 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-ApoER2 Monoclonal Antibody
SKU/Catalog Number
M03444
BM5295 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ApoER2 Monoclonal Antibody catalog # M03444. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ApoER2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03444)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACFH-12
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ApoER2
Reactive Species
M03444 is reactive to LRP8 in Human, Mouse, Rat
Observed Molecular Weight
106 kDa
Calculated molecular weight
105.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03444 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IP 1:20
Validation Images & Assay Conditions
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Western blot analysis of ApoER2 using anti-ApoER2 antibody (M03444).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U251 whole cell lysates,
Lane 2: human U-87MG whole cell lysates,
Lane 3: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ApoER2 antigen affinity purified monoclonal antibody (Catalog # M03444) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ApoER2 at approximately 106 kDa. The expected band size for ApoER2 is at 106 kDa.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Western blot analysis of ApoER2 expression in C6 cell lysate.
Specific Publications For Anti-ApoER2 Monoclonal Antibody (M03444)
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6 Customer Q&As for Anti-ApoER2 Monoclonal Antibody
Question
I was wanting to use your anti-ApoER2 Monoclonal antibody for WB for rat eye lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat eye lung identification?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
You can see on the product datasheet, M03444 anti-ApoER2 Monoclonal antibody has been validated for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat eye lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-27
Question
Is there a BSA free version of anti-ApoER2 Monoclonal antibody M03444 available?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ApoER2 Monoclonal antibody M03444 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-25
Question
See attached the WB image, lot number and protocol we used for eye lung using anti-ApoER2 Monoclonal antibody M03444. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-12
Question
I have a question about product M03444, anti-ApoER2 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Jha
Verified customer
Asked: 2017-08-31
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03444 anti-ApoER2 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-08-31
Question
We are currently using anti-ApoER2 Monoclonal antibody M03444 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
T. Huang
Verified customer
Asked: 2014-09-03
Answer
The anti-ApoER2 Monoclonal antibody (M03444) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-09-03
Question
Is a blocking peptide available for product anti-ApoER2 Monoclonal antibody (M03444)?
P. Collins
Verified customer
Asked: 2014-07-18
Answer
We do provide the blocking peptide for product anti-ApoER2 Monoclonal antibody (M03444). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2014-07-18
