Anti-Apolipoprotein B/APOB Antibody Picoband™

APOB antibody

Boster Bio Anti-Apolipoprotein B/APOB Antibody Picoband™ catalog # PB10042. Tested in IHC, WB applications. This antibody reacts with Human. Cited in 2 publication(s).

Product Info Summary

SKU: PB10042
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-Apolipoprotein B/APOB Antibody Picoband™

View all APOB Antibodies

SKU/Catalog Number

PB10042

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Apolipoprotein B/APOB Antibody Picoband™ catalog # PB10042. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Apolipoprotein B/APOB Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10042)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human Apolipoprotein B recombinant protein (Position: Q246-N450). Human Apolipoprotein B shares 82.4% and 80.5% amino acid (aa) sequence identity with mouse and rat Apolipoprotein B, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB10042 is reactive to APOB in Human

Applications

PB10042 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

250 kDa

Calculated molecular weight

515.605kDa

Background of APOB

Apolipoprotein B (ApoB) is a protein that in humans is encoded by the APOB gene. This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For APOB (Source: Uniprot.org, NCBI)

Gene Name

APOB

Full Name

Apolipoprotein B-100

Weight

515.605kDa

Alternative Names

Apo B-100; APOB; ApoB-100; ApoB-48; apolipoprotein B (including Ag(x) antigen); Apolipoprotein B; apolipoprotein B-100; apolipoprotein B48; FLDB; LDLCQ4; mutant Apo B 100 APOB FCHL2, FLDB, LDLCQ4, apoB-100, apoB-48 apolipoprotein B apolipoprotein B-100|apolipoprotein B (including Ag(x) antigen)|apolipoprotein B48

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on APOB, check out the APOB Infographic

APOB infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for APOB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB10042 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Huang Y,Zhao C,Kong Y,Tan P,Liu S,Liu Y,Zeng F,Yuan Y,Zhao B,Wang J.Elucidation of the mechanism of NEFA-induced PERK-eIF2α signaling pathway regulation of lipid metabolism in bovine hepatocytes.J Steroid Biochem Mol Biol.2021 Apr 2:105893.doi:10.101 6/j.jsbmb.2021.105893.Epub ahead of print.PMID:33819629.
Species: Holstein calves
PB10042 usage in article: APP:WB, SAMPLE:HEPATOCYTES, DILUTION:1:1000

Kong Y, Zhao C, Huang Y, Liu Y, Liu S, Guo Y, Li M, Xu T, Zhao B, Wang J. Angiopoietin-like protein 4 promotes very-low-density lipoprotein assembly and secretion in bovine hepatocytes in vitro. IUBMB Life. 2020 Nov 17. doi: 10.1002/iub.2403. Epub ahead o
Species: Calf

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4 Customer Q&As for Anti-Apolipoprotein B/APOB Antibody Picoband™

Question

We are currently using anti-Apolipoprotein B/APOB antibody PB10042 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-26

Answer

The anti-Apolipoprotein B/APOB antibody (PB10042) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-26

Question

Will anti-Apolipoprotein B/APOB antibody PB10042 work on horse WB with small intestine?

Verified Customer

Verified customer

Asked: 2019-06-20

Answer

Our lab technicians have not tested anti-Apolipoprotein B/APOB antibody PB10042 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Apolipoprotein B/APOB antibody PB10042 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse small intestine in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-20

Question

I am interested in to test anti-Apolipoprotein B/APOB antibody PB10042 on human jejunal mucosa for research purposes, then I may be interested in using anti-Apolipoprotein B/APOB antibody PB10042 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

G. Zhao

Verified customer

Asked: 2018-12-12

Answer

The products we sell, including anti-Apolipoprotein B/APOB antibody PB10042, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-12-12

Question

Would anti-Apolipoprotein B/APOB antibody PB10042 work for IHC with jejunal mucosa?

Verified Customer

Verified customer

Asked: 2018-10-26

Answer

According to the expression profile of jejunal mucosa, APOB is highly expressed in jejunal mucosa. So, it is likely that anti-Apolipoprotein B/APOB antibody PB10042 will work for IHC with jejunal mucosa.

Boster Scientific Support

Answered: 2018-10-26

Order DetailsPrice
PB10042

100μg

$370
PB10042-10ug

10μg sample (liquid)

$99
PB10042-Biotin

100 μg Biotin conjugated

$570
PB10042-Cy3

100 μg Cy3 conjugated

$570
PB10042-Dylight488

100 μg Dylight488 conjugated

$570
PB10042-Dylight550

100 μg Dylight550 conjugated

$570
PB10042-Dylight594

100 μg Dylight594 conjugated

$570
PB10042-FITC

100 μg FITC conjugated

$570
PB10042-HRP

100 μg HRP conjugated

$570
PB10042-APC

100 μg APC conjugated

$670
PB10042-PE

100 μg PE conjugated

$670
PB10042-carrier-free

Carrier Free

$370

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