Anti-APOLIPOPROTEINC-III APOC3 Antibody

Product Name

Anti-APOLIPOPROTEINC-III APOC3 Antibody

View all APOC3 Antibodies

SKU/Catalog Number

A01416

Size

1mg

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-APOLIPOPROTEINC-III APOC3 Antibody (Catalog # A01416). Tested in IHC applications. This antibody reacts with Human.

Storage & Handling

Store vial at 4°C prior to opening. This product is stable 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening. (Ship on wet ice.)

Cite This Product

Anti-APOLIPOPROTEINC-III APOC3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01416)

Host

Goat

Contents

0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

produced from apoLipoprotein Type C-III derived from human plasma

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

A01416 is reactive to APOC3 in Human

Observed Molecular Weight

68 kDa

Calculated molecular weight

10.9 kDa

Background of APOC3

Anti Apolipoprotein C-III antibody recognizes the gene product of APOC. Apolipoprotein C-III is a protein component of very low density lipoprotein (VLDL). APOC3 inhibits lipoprotein lipase and hepatic lipase; it is thought to inhibit hepatic uptake of triglyceride-rich particles. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the A-1 and C-III genes are convergently transcribed. An increase in apoC-III levels induces the development of hypertriglyceridemia. This antibody is suitable for cardiovascular research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A01416 is guaranteed for ELISA, IP, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5,000 - 1:10,000
IHC: 1:50 - 1:200
IP: 1:100
WB: 1:500 - 1:5,000
Anti-Apolipoprotein antibodies have been tested in immunohistochemistry and used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, immunohistochemistry, and for western blotting for highly sensitive qualitative analysis. When performing immunoblotting assays - do not block with blotto. Blotto formulations tend to contain apoLipoprotein Type C-III and will neutralize antibody activity.

Validation Images & Assay Conditions

See full target information APOC3

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis