Product Info Summary
SKU: | M02381 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Mouse |
Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-APPL/APPL1 Antibody Picoband™ (monoclonal, 5G11)
SKU/Catalog Number
M02381
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-APPL/APPL1 Antibody Picoband™ (monoclonal, 5G11) catalog # M02381. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-APPL/APPL1 Antibody Picoband™ (monoclonal, 5G11) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02381)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Monoclonal
Clone Number
5G11
Isotype
Mouse IgG2a
Immunogen
E.coli-derived human APPL/APPL1 recombinant protein (Position: K91-R668).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M02381 is reactive to APPL1 in Human, Mouse, Rat
Applications
M02381 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
85 kDa
Calculated molecular weight
79.663kDa
Background of APPL
DCC-interacting protein 13-alpha (APPL1) is a protein that in humans is encoded by the APPL1 gene. The APPL1 gene is mapped to 3q21.1-p13.3. It is said to contain 709 amino acids and share 54% amino acid identity with APPL2. APPL is highly expressed in skeletal muscle, heart, ovary, and pancreas, tissues in which AKT2 mRNA is abundant. It has been regarded as an adaptor that may tether inactive AKT2 to the PI3K in the cytoplasm and thereby may expedite recruitment of AKT2 and PI3K to the cell membrane upon mitogenic stimulation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of APPL/APPL1 using anti-APPL/APPL1 antibody (M02381).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-APPL/APPL1 antigen affinity purified monoclonal antibody (Catalog # M02381) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for APPL/APPL1 at approximately 85KD. The expected band size for APPL/APPL1 is at 85KD.
Click image to see more details
Figure 2. IHC analysis of APPL/APPL1 using anti-APPL/APPL1 antibody (M02381).
APPL/APPL1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-APPL/APPL1 Antibody (M02381) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of APPL/APPL1 using anti-APPL/APPL1 antibody (M02381).
APPL/APPL1 was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-APPL/APPL1 Antibody (M02381) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of APPL/APPL1 using anti-APPL/APPL1 antibody (M02381).
APPL/APPL1 was detected in paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-APPL/APPL1 Antibody (M02381) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 5. Flow Cytometry analysis of U-937 cells using anti-APPL/APPL1 antibody (M02381).
Overlay histogram showing U-937 cells stained with M02381 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-APPL/APPL1 Antibody (M02381, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For APPL1 (Source: Uniprot.org, NCBI)
Gene Name
APPL1
Full Name
DCC-interacting protein 13-alpha
Weight
79.663kDa
Alternative Names
Adapter protein containing PH domain, PTB domain and leucine zipper motif 1; adaptor protein containing pH domain, PTB domain and leucine zipper motif 1; adaptor protein, phosphotyrosine interaction, PH domain and leucine zippercontaining 1; AKT2 interactor; APPL; APPL1; APPLdip13-alpha; DCC-interacting protein 13-alpha; DIP 13 alpha; DIP13A; DIP13alpha; Dip13-alpha; KIAA1428; signaling adaptor protein DIP13alpha APPL1 APPL, DIP13alpha, MODY14 adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 DCC-interacting protein 13-alpha|AKT2 interactor|adapter protein containing PH domain, PTB domain and leucine zipper motif 1|adaptor protein containing pH domain, PTB domain and leucine zipper motif 1|adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1|dip13-alpha|signaling adaptor protein DIP13alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on APPL1, check out the APPL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for APPL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-APPL/APPL1 Antibody Picoband™ (monoclonal, 5G11) (M02381)
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No publications found for M02381
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