Click image to see more details
Product Info Summary
| SKU: | PB9436 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Glucose Transporter GLUT2/SLC2A2 Antibody Picoband®
SKU/Catalog Number
PB9436
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Glucose Transporter GLUT2/SLC2A2 Antibody Picoband® catalog # PB9436. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Glucose Transporter GLUT2/SLC2A2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9436)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SLC2A2, different from the related mouse sequence by five amino acids, and from the related rat sequence by seven amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9436 is reactive to SLC2A2 in Human
Observed Molecular Weight
50 kDa
Calculated molecular weight
57.5 kDa
Background of SLC2A2
SLC2A2, also known as Glucose transporter 2 (GLUT2), is a transmembrane carrier protein that enables protein facilitatedglucose movement across cell membranes. This gene encodes an integral plasma membrane glycoprotein of the liver, islet beta cells, intestine, and kidney epithelium. The encoded protein mediates facilitated bidirectional glucose transport. Because of its low affinity for glucose, it has been suggested as a glucose sensor. Mutations in this gene are associated with susceptibility to diseases, including Fanconi-Bickel syndrome and noninsulin-dependent diabetes mellitus (NIDDM). Alternative splicing results in multiple transcript variants of this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9436 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: PANC Whole Cell, A549 Whole Cell, HT1080 Whole Cell
IHC: human liver cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of SLC2A2 using anti-SLC2A2 antibody (PB9436).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: PANC Whole Cell Lysate at 40ug,
Lane 2: A549 Whole Cell Lysate at 40ug,
Lane 3: HT1080 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC2A2 antigen affinity purified polyclonal antibody (Catalog # PB9436) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC2A2 at approximately 50 kDa. The expected band size for SLC2A2 is at 57 kDa.
Click image to see more details
IHC analysis of SLC2A2 using anti-SLC2A2 antibody (PB9436).
SLC2A2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SLC2A2 Antibody (PB9436) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-Glucose Transporter GLUT2/SLC2A2 Antibody Picoband® (PB9436)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Glucose Transporter GLUT2/SLC2A2 Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Glucose Transporter GLUT2/SLC2A2 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
1 Customer Q&As for Anti-Glucose Transporter GLUT2/SLC2A2 Antibody Picoband®
Question
We are currently using anti-Glucose Transporter GLUT2/SLC2A2 antibody PB9436 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
J. Edwards
Verified customer
Asked: 2019-07-26
Answer
The anti-Glucose Transporter GLUT2/SLC2A2 antibody (PB9436) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-26
