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Product Info Summary
| SKU: | M03085 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-AQP5 Rabbit Monoclonal Antibody
SKU/Catalog Number
M03085
BM4433 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-AQP5 Rabbit Monoclonal Antibody catalog # M03085. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AQP5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03085)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
EFG-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human AQP5
Reactive Species
M03085 is reactive to AQP5 in Human
Observed Molecular Weight
28 kDa
Calculated molecular weight
28.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03085 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
WB: SW480 cell
IHC: mouse lung tissue
Validation Images & Assay Conditions
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Western blot analysis of AQP5 expression in SW480 cell lysate.
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Immunohistochemical analysis of paraffin-embedded mouse lung, using AQP5 Antibody.
Specific Publications For Anti-AQP5 Rabbit Monoclonal Antibody (M03085)
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Customer Q&As
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5 Customer Q&As for Anti-AQP5 Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-AQP5 Rabbit Monoclonal antibody for WB for human right testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human right testis identification?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
As indicated on the product datasheet, M03085 anti-AQP5 Rabbit Monoclonal antibody has been tested for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human right testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-22
Question
I have left two msgs to return my call with this question last two days but did not get any response.
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
The immunogen sequence of M03085 is aa245-265. It shares 78.9% sequence homology with mouse aqp5. Abways is uncertain if the signal at 20kD represents Aqp5. They haven't experimentally validate this.
Boster Scientific Support
Answered: 2019-09-18
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right testis using anti-AQP5 Rabbit Monoclonal antibody M03085. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-19
Question
Do you have a BSA free version of anti-AQP5 Rabbit Monoclonal antibody M03085 available?
L. Wu
Verified customer
Asked: 2018-05-31
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-AQP5 Rabbit Monoclonal antibody M03085 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-05-31
Question
I have attached the WB image, lot number and protocol we used for right testis using anti-AQP5 Rabbit Monoclonal antibody M03085. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-09-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-13
