Anti-Aquaporin 10/AQP10 Antibody

Aquaporin-10 antibody

Boster Bio Anti-Aquaporin 10/AQP10 Antibody catalog # PA2095. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: PA2095
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-Aquaporin 10/AQP10 Antibody

View all Aquaporin-10 Antibodies

SKU/Catalog Number

PA2095

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Aquaporin 10/AQP10 Antibody catalog # PA2095. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Aquaporin 10/AQP10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2095)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 10.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2095 is reactive to AQP10 in Human

Applications

PA2095 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

31 kDa

Calculated molecular weight

31.763kDa

Background of Aquaporin-10

AQP10 was identified in human small intestine. This gene encoded a 264-amino-acid protein with high sequence identity with AQP3 (53%), 9 (52%), and 7 (43%). These AQPs constitute one subfamily of AQP family that is differentiated from the other subfamily of AQP (AQP0, 1, 2, 4, 5, 6, and 8) by sequence homology. Northern blot analysis revealed expression of a 2.3-kb AQP10 transcript in jejunum but not liver. RNase protection analysis detected nearly exclusive expression in duodenum and jejunum. In situ hybridization analysis demonstrated highest expression of AQP10 in absorptive epithelial cells at the tips of villi in the jejunum. SDS-PAGE analysis showed expression of a 28-kD AQP10 protein, similar to its predicted size as well as to that of the other AQPs. Functional analysis indicated that cells expressing AQP10 are permeable to relatively low amounts of water, but, unlike AQP3, AQP7, and AQP9, are not permeable to urea or glycerol.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For AQP10 (Source: Uniprot.org, NCBI)

Gene Name

AQP10

Full Name

Aquaporin-10

Weight

31.763kDa

Superfamily

MIP/aquaporin (TC 1.A.8) family

Alternative Names

AQP-10; AQPA_HUMAN; aquaglyceroporin-10; aquaporin 10; aquaporin-10; Small intestine aquaporin AQP10 AQPA_HUMAN aquaporin 10 aquaporin-10|AQP-10|aquaglyceroporin-10|small intestine aquaporin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on AQP10, check out the AQP10 Infographic

AQP10 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for AQP10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-Aquaporin 10/AQP10 Antibody

Question

Can you help my question with product PA2095, anti-Aquaporin 10/AQP10 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-11-26

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2095 anti-Aquaporin 10/AQP10 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-11-26

Question

We are currently using anti-Aquaporin 10/AQP10 antibody PA2095 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?

F. Parker

Verified customer

Asked: 2019-08-27

Answer

The anti-Aquaporin 10/AQP10 antibody (PA2095) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-27

Question

Will anti-Aquaporin 10/AQP10 antibody PA2095 work on pig WB with jejunal mucosa?

Verified Customer

Verified customer

Asked: 2019-06-25

Answer

Our lab technicians have not validated anti-Aquaporin 10/AQP10 antibody PA2095 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Aquaporin 10/AQP10 antibody PA2095 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig jejunal mucosa in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-25

Question

Is there a BSA free version of anti-Aquaporin 10/AQP10 antibody PA2095 available?

J. Jha

Verified customer

Asked: 2018-08-16

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Aquaporin 10/AQP10 antibody PA2095 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-08-16

Question

See below the WB image, lot number and protocol we used for lung using anti-Aquaporin 10/AQP10 antibody PA2095. Please let me know if you require anything else.

B. Walker

Verified customer

Asked: 2013-04-23

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-04-23

Order DetailsPrice
PA2095

100μg

$370
PA2095-10ug

10μg sample (liquid)

$99
PA2095-Biotin

100 μg Biotin conjugated

$570
PA2095-Cy3

100 μg Cy3 conjugated

$570
PA2095-Dylight488

100 μg Dylight488 conjugated

$570
PA2095-Dylight550

100 μg Dylight550 conjugated

$570
PA2095-Dylight594

100 μg Dylight594 conjugated

$570
PA2095-FITC

100 μg FITC conjugated

$570
PA2095-HRP

100 μg HRP conjugated

$570
PA2095-APC

100 μg APC conjugated

$670
PA2095-PE

100 μg PE conjugated

$670
PA2095-carrier-free

Carrier Free

$370

More conjugates/formats

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PA2095
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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