Anti-Aquaporin 4/AQP4 Antibody

Aquaporin-4 antibody

Boster Bio Anti-Aquaporin 4/AQP4 Antibody catalog # PA1220. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 6 publication(s).

Product Info Summary

SKU: PA1220
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Customers Who Bought This Also Bought

Product Name

Anti-Aquaporin 4/AQP4 Antibody

View all Aquaporin-4 Antibodies

SKU/Catalog Number



100 μg/vial




Boster Bio Anti-Aquaporin 4/AQP4 Antibody catalog # PA1220. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Aquaporin 4/AQP4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1220)




Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.




Rabbit IgG


A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 4 (172-188aa FTIFASCDSKRTDVTGS), identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.


No cross-reactivity with other proteins

Reactive Species

PA1220 is reactive to AQP4 in Human, Mouse, Rat


PA1220 is guaranteed for WB Boster Guarantee

Background of Aquaporin-4

Aquaporin 4 is found in the basolateral cell membrane of principal collecting duct cells and provide a pathway for water to exit these cells. The gene of AQP4 is mapped to 18q11.2-q12.1.Similar to other aquaporins, the AQP4 gene is composed of 4 exons encoding 127, 55, 27, and 92 amino acids separated by introns of 0.8, 0.3, and 5.2 kb. Unlike other aquaporins, an alternative coding initiation sequence (designated exon 0) was located 2.7 kb upstream of exon 1. When spliced together, M1 and the subsequent 10 amino acids are encoded by exon 0; the next 11 amino acids and M23 are encoded by exon 1. AQP4 is expressed in astrocytes and is upregulated by direct insult to the central nervous system. AQP4 is the predominant water channel in the brain and has an important role in brain water homeostasis. It is abundant in mammalian brain and is concentrated in astrocytic foot processes at the blood-brain barrier.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review


Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For AQP4 (Source:, NCBI)

Uniprot ID


Gene ID


Gene Name


Full Name





MIP/aquaporin (TC 1.A.8) family

Alternative Names

aquaporin 4; MGC22454 AQP4 MIWC, WCH4 aquaporin 4 aquaporin-4|aquaporin type4|mercurial-insensitive water channel

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on AQP4, check out the AQP4 Infographic

AQP4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for AQP4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

PA1220 has been cited in 6 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Effects of aquaporin 4 and inward rectifier potassium channel 4.1 on medullospinal edema after methylprednisolone treatment to suppress acute spinal cord injury in rats

Changes in the Expression of AQP4 and AQP9 in the Hippocampus Following Eclampsia-Like Seizure

Expression pattern of aquaporins in patients with primary nephrotic syndrome with edema

Neuroprotective effects of total steroid saponins on cerebral ischemia injuries in animal model of focal ischemia/reperfusion

Anzabi M, Ardalan M, Iversen NK, Rafati AH, Hansen B, ?stergaard L. Front Cell Neurosci. 2018 Jan 31;12:19. doi: 10.3389/fncel.2018.00019. eCollection 2018. Hippocampal Atrophy Following Subarachnoid Hemorrhage Correlates with Disruption of Astroc...

Chen Hm, Huang Hs, Ruan L, He Yb, Li Xj. Int J Clin Exp Med. 2014 May 15;7(5):1483-9. Ecollection 2014. Ulinastatin Attenuates Cerebral Ischemia-Reperfusion Injury In Rats.

Have you used Anti-Aquaporin 4/AQP4 Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-Aquaporin 4/AQP4 Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

5 Customer Q&As for Anti-Aquaporin 4/AQP4 Antibody


We are currently using anti-Aquaporin 4/AQP4 antibody PA1220 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-25


The anti-Aquaporin 4/AQP4 antibody (PA1220) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-25


I would like using your anti-Aquaporin 4/AQP4 antibody for water homeostasis studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-07-05


We appreciate your inquiry. This PA1220 anti-Aquaporin 4/AQP4 antibody is tested on rat heart tissue, tissue lysate, brain tissue, colo320 cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-07-05


My team were happy with the WB result of your anti-Aquaporin 4/AQP4 antibody. However we have observed positive staining in fetal brain cell membrane using this antibody. Is that expected? Could you tell me where is AQP4 supposed to be expressed?

C. Zhang

Verified customer

Asked: 2017-05-24


According to literature, fetal brain does express AQP4. Generally AQP4 expresses in cell membrane. Regarding which tissues have AQP4 expression, here are a few articles citing expression in various tissues:
Fetal brain, Pubmed ID: 7559426
Lung, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-05-24


I have seen bands on my Western Blot even after blocking the assay with the corresponding 10X peptide (Boster Catalog# BP1220). I am concerned about the specificity of the antibody and would like to know how it has been validated. Do you have a good positive and negative control for cell lysates?

Verified Customer

Verified customer

Asked: 2015-04-29


First of all, we suggest you use a 100X peptide. Secondly, this gene is highly expressed in brain tissue (human, mouse, rat), so use Hele, MCF-7 or COLO cell in brain tissue as your positive control. For negative control, take kidney, lung or spleen tissue. However, its expression is very low.

Boster Scientific Support

Answered: 2015-04-29


We have observed staining in mouse lateral globus pallidus. Are there any suggestions? Is anti-Aquaporin 4/AQP4 antibody supposed to stain lateral globus pallidus positively?

G. Rodriguez

Verified customer

Asked: 2015-04-15


From what I have seen in literature lateral globus pallidus does express AQP4. From what I have seen in, AQP4 is expressed in lateral globus pallidus, fetal brain, lung, among other tissues. Regarding which tissues have AQP4 expression, here are a few articles citing expression in various tissues:
Fetal brain, Pubmed ID: 7559426
Lung, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2015-04-15



Total: $315


Ask a question

Get A Quote

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)

$50 fee for conjugation. Antibody size is reduced to 50ug.



Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.