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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Anti-Aquaporin 4/AQP4 Antibody
Boster Bio Anti-Aquaporin 4/AQP4 Antibody catalog # PA1220. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Aquaporin 4/AQP4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1220)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 4 (172-188aa FTIFASCDSKRTDVTGS), identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins
PA1220 is reactive to AQP4 in Human, Mouse, Rat
PA1220 is guaranteed for WB Boster Guarantee
Background of Aquaporin-4
Aquaporin 4 is found in the basolateral cell membrane of principal collecting duct cells and provide a pathway for water to exit these cells. The gene of AQP4 is mapped to 18q11.2-q12.1.Similar to other aquaporins, the AQP4 gene is composed of 4 exons encoding 127, 55, 27, and 92 amino acids separated by introns of 0.8, 0.3, and 5.2 kb. Unlike other aquaporins, an alternative coding initiation sequence (designated exon 0) was located 2.7 kb upstream of exon 1. When spliced together, M1 and the subsequent 10 amino acids are encoded by exon 0; the next 11 amino acids and M23 are encoded by exon 1. AQP4 is expressed in astrocytes and is upregulated by direct insult to the central nervous system. AQP4 is the predominant water channel in the brain and has an important role in brain water homeostasis. It is abundant in mammalian brain and is concentrated in astrocytic foot processes at the blood-brain barrier.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Validation Images & Assay Conditions
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Anti-Aquaporin 4 antibody, PA1220, Western blotting
Lane 1: Rat Heart Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: COLO320 Cell Lysate
Gene/Protein Information For AQP4 (Source: Uniprot.Org, NCBI)
MIP/aquaporin (TC 1.A.8) family
aquaporin 4; MGC22454 AQP4 MIWC, WCH4 aquaporin 4 aquaporin-4|aquaporin type4|mercurial-insensitive water channel*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on AQP4, check out the AQP4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for AQP4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
PA1220 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Changes in the Expression of AQP4 and AQP9 in the Hippocampus Following Eclampsia-Like Seizure
Expression pattern of aquaporins in patients with primary nephrotic syndrome with edema
Neuroprotective effects of total steroid saponins on cerebral ischemia injuries in animal model of focal ischemia/reperfusion
Anzabi M, Ardalan M, Iversen NK, Rafati AH, Hansen B, ?stergaard L. Front Cell Neurosci. 2018 Jan 31;12:19. doi: 10.3389/fncel.2018.00019. eCollection 2018. Hippocampal Atrophy Following Subarachnoid Hemorrhage Correlates with Disruption of Astroc...
Chen Hm, Huang Hs, Ruan L, He Yb, Li Xj. Int J Clin Exp Med. 2014 May 15;7(5):1483-9. Ecollection 2014. Ulinastatin Attenuates Cerebral Ischemia-Reperfusion Injury In Rats.
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