Anti-Aquaporin 4/AQP4 Antibody
Rabbit IgG polyclonal antibody for Aquaporin-4(AQP4) detection. Tested with WB in Human;Mouse;Rat. Cited in 5 publication(s).
Anti-Aquaporin 4/AQP4 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-Aquaporin 4/AQP4 Antibody
See all Aquaporin-4 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for AQUAPORIN 4/AQP4 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. AQUAPORIN 4/AQP4 information: Molecular Weight: 34830 MW; Subcellular Localization: Membrane; Multi-pass membrane protein; Tissue Specificity: Brain - muscle >> heart, kidney, lung, and trachea.|
|Cite This Product||Anti-Aquaporin 4/AQP4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1220)|
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|Anti-Aquaporin 4/AQP4 Antibody may replace the following items: sc 20812|sc 9887|sc 9888|sc 32739|sc 390636|sc 390488.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 4(172-188aa FTIFASCDSKRTDVTGS), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For AQP4 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||361|
|Species Of This Entry||Human|
|Superfamily||MIP/aquaporin (TC 1.A.8) family|
|Alternative Names||Aquaporin-4|aquaporin 4; MGC22454|
|Post Tranlational Modifications||Phosphorylation; Glycosylation; Biotinylation; Reduction; Palmitoylation; Ubiquitination; Nitration; Dephosphorylation|
|Subcellular Localization||Cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein. Endosome membrane. Cell membrane, sarcolemma; Multi-pass membrane protein. Cell projection. Activation of the vasopressin receptor AVPR1A triggers AQP4 phosphorylation at Ser-180 and promotes its internalization from the cell membrane. Detected on brain astrocyte processes and astrocyte endfeet close to capillaries.|
|Gene Location||18q11.2, on Chromasome 18, gene sequence: NC_000018.10 (26852038..26865803, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For AQP4 (Source: Uniprot.org, NCBI)
|Protein Function||Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.|
|Tissue Specificity||Brain - muscle >> heart, kidney, lung, and trachea.|
|Related Diseases||Edema; Neuromyelitis Optica; Brain Edema; Nervousness; Multiple Sclerosis; Sclerosis; Myelitis; Optic Neuritis; Neuritis; Demyelinating Diseases; Brain Injuries; Autoimmune Reaction; Ischemia; Cerebral Edema; Cerebrovascular Accident; Myelitis, Transverse; Neoplasms; Cns Disorder; Inflammation|
|Related Pathways||Transport; Water Transport; Localization; Pathogenesis; Water Homeostasis; Secretion; Fluid Transport; Ion Homeostasis; Cell Migration; Cell Death; Angiogenesis; Cellular Localization; Complement Activation; Cell Adhesion; Neuroprotection; Translation; Regeneration; Neurogenesis; Reverse Transcription; Endocytosis|
|Background||Aquaporin 4 is found in the basolateral cell membrane of principal collecting duct cells and provide a pathway for water to exit these cells. The gene of AQP4 is mapped to 18q11.2-q12.1.Similar to other aquaporins, the AQP4 gene is composed of 4 exons encoding 127, 55, 27, and 92 amino acids separated by introns of 0.8, 0.3, and 5.2 kb. Unlike other aquaporins, an alternative coding initiation sequence(designated exon 0) was located 2.7 kb upstream of exon 1. When spliced together, M1 and the subsequent 10 amino acids are encoded by exon 0; the next 11 amino acids and M23 are encoded by exon 1. AQP4 is expressed in astrocytes and is upregulated by direct insult to the central nervous system. AQP4 is the predominant water channel in the brain and has an important role in brain water homeostasis. It is abundant in mammalian brain and is concentrated in astrocytic foot processes at the blood-brain barrier.|
|Scientific References||PMID: 7559426 by Yang B., et al. cDNA cloning, gene organization, and chromosomal localization of a human mercurial insensitive water channel. Evidence for distinct transcriptional units.|
PMID: 8601457 by Misaka T., et al. A water channel closely related to rat brain aquaporin 4 is expressed in acid- and pepsinogen-secretory cells of human stomach.
Our Boster Quality Guarantee for Anti-Aquaporin 4/AQP4 Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Rat Heart Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: COLO320 Cell Lysate
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Total number of citations: 5
|Chen Hm, Huang Hs, Ruan L, He Yb, Li Xj. Int J Clin Exp Med. 2014 May 15;7(5):1483-9. Ecollection 2014. Ulinastatin Attenuates Cerebral Ischemia-Reperfusion Injury In Rats.||PubMed ID 24995117|
|Anzabi M, Ardalan M, Iversen NK, Rafati AH, Hansen B, ?stergaard L. Front Cell Neurosci. 2018 Jan 31;12:19. doi: 10.3389/fncel.2018.00019. eCollection 2018. Hippocampal Atrophy Following Subarachnoid Hemorrhage Correlates with Disruption of Astroc...||PubMed ID 29445328|
|Neuroprotective effects of total steroid saponins on cerebral ischemia injuries in animal model of focal ischemia/reperfusion||PubMed ID 24963614|
|Expression pattern of aquaporins in patients with primary nephrotic syndrome with edema||PubMed ID 26261083|
|Changes in the Expression of AQP4 and AQP9 in the Hippocampus Following Eclampsia-Like Seizure||PubMed ID 29351212|
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