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Product Info Summary
| SKU: | M01049 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Aquaporin 4 AQP4 Monoclonal Antibody
SKU/Catalog Number
M01049
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Aquaporin 4 AQP4 Monoclonal Antibody catalog # M01049. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Aquaporin 4 AQP4 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01049)
Host
Mouse
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
4H1
Isotype
IgG
Immunogen
Synthetic Peptide of Aquaporin 4
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M01049 is reactive to AQP4 in Human, Mouse, Rat
Calculated molecular weight
34.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01049 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000
IF 1:100-200
IHC 1:50-300
Validation Images & Assay Conditions
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Western blot analysis of lysates from 1) Hela, 2) Mouse Heart tissue, 3) Rat Heart Tissue cells, (Green) primary antibody was diluted at 1:1000, 4°over night, secondary antibody was diluted at 1:10000, 37° 1hour. (Red) GAPDH Polyclonal Antibody was diluted at 1:5000 as loading control, 4° over night, secondary antibody was diluted at 1:10000, 37° 1hour.
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Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, Aquaporin 4 Monoclonal Antibody (4H1) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
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IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.
Specific Publications For Anti-Aquaporin 4 AQP4 Monoclonal Antibody (M01049)
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