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Product Info Summary
| SKU: | PB9475 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Aquaporin 4/AQP4 Antibody Picoband™
View all Aquaporin-4 Antibodies
SKU/Catalog Number
PB9475
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Aquaporin 4/AQP4 Antibody Picoband™ catalog # PB9475. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Aquaporin 4/AQP4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9475)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 4, different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9475 is reactive to AQP4 in Human, Mouse, Rat
Applications
PB9475 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
37 kDa
Calculated molecular weight
34.83kDa
Background of Aquaporin-4
Aquaporin 4 is found in the basolateral cell membrane of principal collecting duct cells and provides a pathway for water to exit these cells. The gene of AQP4 is mapped to 18q11.2-q12.1. Similar to other aquaporins, the AQP4 gene is composed of 4 exons encoding 127, 55, 27, and 92 amino acids separated by introns of 0.8, 0.3, and 5.2 kb. Unlike other aquaporins, an alternative coding initiation sequence (designated exon 0) was located 2.7 kb upstream of exon 1. When spliced together, M1 and the subsequent 10 amino acids are encoded by exon 0; the next 11 amino acids and M23 are encoded by exon 1. AQP4 is expressed in astrocytes and is upregulated by direct insult to the central nervous system. And AQP4 is the predominant water channel in the brain and has an important role in brain water homeostasis. It is abundant in mammalian brain and is concentrated in astrocytic foot processes at the blood-brain barrier.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Aquaporin 4 using anti-Aquaporin 4 antibody (PB9475).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aquaporin 4 antigen affinity purified polyclonal antibody (Catalog # PB9475) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aquaporin 4 at approximately 37 kDa. The expected band size for Aquaporin 4 is at 35 kDa.
Click image to see more details
Figure 2. IHC analysis of Aquaporin 4 using anti-Aquaporin 4 antibody (PB9475).
Aquaporin 4 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Aquaporin 4 Antibody (PB9475) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Aquaporin 4 using anti-Aquaporin 4 antibody (PB9475).
Aquaporin 4 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Aquaporin 4 Antibody (PB9475) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Aquaporin 4 using anti-Aquaporin 4 antibody (PB9475).
Aquaporin 4 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Aquaporin 4 Antibody (PB9475) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of Aquaporin 4 using anti-Aquaporin 4 antibody (PB9475).
Aquaporin 4 was detected in frozen section of Mouse Brain Tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Aquaporin 4 Antibody (PB9475) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of Aquaporin 4 using anti-Aquaporin 4 antibody (PB9475).
Aquaporin 4 was detected in frozen section of Rat Brain Tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Aquaporin 4 Antibody (PB9475) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For AQP4 (Source: Uniprot.org, NCBI)
Gene Name
AQP4
Full Name
Aquaporin-4
Weight
34.83kDa
Superfamily
MIP/aquaporin (TC 1.A.8) family
Alternative Names
aquaporin 4; MGC22454 AQP4 MIWC, WCH4 aquaporin 4 aquaporin-4|aquaporin type4|mercurial-insensitive water channel
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AQP4, check out the AQP4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AQP4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Aquaporin 4/AQP4 Antibody Picoband™ (PB9475)
Hello CJ!
PB9475 has been cited in 9 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Brain edema after intracerebral hemorrhage in rats: the role of iron overload and aquaporin 4: Laboratory investigation
Group 2 innate lymphoid cells suppress the pathology of neuromyelitis optica spectrum disorder
Expression of AQP1, AQP3, AQP4 and AQP5 in upper respiratory tract of buffaloes during different seasons
Song T,Chen W,Chen X,Zhang H,Zou Y,Wu H,Lin F,Ren L,Kang Y,Lei H.Repeated fluoxetine treatment induces transient and long-term astrocytic plasticity in the medial prefrontal cortex of normal adult rats.Prog Neuropsychopharmacol Biol Psychiatry.2021 Jan 20
Species: Rat
PB9475 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:3000
Changes in the Expression of AQP4 and AQP9 in the Hippocampus Following Eclampsia-Like Seizure
Expression pattern of aquaporins in patients with primary nephrotic syndrome with edema
Neuroprotective effects of total steroid saponins on cerebral ischemia injuries in animal model of focal ischemia/reperfusion
Anzabi M, Ardalan M, Iversen NK, Rafati AH, Hansen B, ?stergaard L. Front Cell Neurosci. 2018 Jan 31;12:19. doi: 10.3389/fncel.2018.00019. eCollection 2018. Hippocampal Atrophy Following Subarachnoid Hemorrhage Correlates with Disruption of Astroc...
Chen Hm, Huang Hs, Ruan L, He Yb, Li Xj. Int J Clin Exp Med. 2014 May 15;7(5):1483-9. Ecollection 2014. Ulinastatin Attenuates Cerebral Ischemia-Reperfusion Injury In Rats.
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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18 Customer Q&As for Anti-Aquaporin 4/AQP4 Antibody Picoband™
Question
Do you have a BSA free version of anti-Aquaporin 4/AQP4 antibody PB9475 available?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Aquaporin 4/AQP4 antibody PB9475 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-05-04
Question
Would anti-Aquaporin 4/AQP4 antibody PB9475 work on primate IHC with lateral globus pallidus?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
Our lab technicians have not validated anti-Aquaporin 4/AQP4 antibody PB9475 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Aquaporin 4/AQP4 antibody PB9475 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate lateral globus pallidus in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-20
Question
I am interested in to test anti-Aquaporin 4/AQP4 antibody PB9475 on mouse lung for research purposes, then I may be interested in using anti-Aquaporin 4/AQP4 antibody PB9475 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
The products we sell, including anti-Aquaporin 4/AQP4 antibody PB9475, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-17
Question
We have been able to see staining in mouse lung. Do you have any suggestions? Is anti-Aquaporin 4/AQP4 antibody supposed to stain lung positively?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
Based on literature lung does express AQP4. Based on Uniprot.org, AQP4 is expressed in lateral globus pallidus, fetal brain, lung, among other tissues. Regarding which tissues have AQP4 expression, here are a few articles citing expression in various tissues:
Fetal brain, Pubmed ID: 7559426
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-01-17
Question
Would PB9475 anti-Aquaporin 4/AQP4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
It shows on the product datasheet, PB9475 anti-Aquaporin 4/AQP4 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-16
Question
Is this PB9475 anti-Aquaporin 4/AQP4 antibody reactive to the isotypes of AQP4?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
The immunogen of PB9475 anti-Aquaporin 4/AQP4 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 4 (295-323aa KPGVVHVIDVDRGEEKKGKDQSGEVLSSV), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-26
Question
We have tried in the past anti-Aquaporin 4/AQP4 antibody for WB on fetal brain a few months ago. I am using mouse, and We are going to use the antibody for IHC next. I am interested in examining fetal brain as well as lateral globus pallidus in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
I looked at the website and datasheets of our anti-Aquaporin 4/AQP4 antibody and it seems that PB9475 has been tested on mouse in both WB and IHC. Thus PB9475 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-11-15
Question
Is a blocking peptide available for product anti-Aquaporin 4/AQP4 antibody (PB9475)?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
We do provide the blocking peptide for product anti-Aquaporin 4/AQP4 antibody (PB9475). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-05
Question
A customer is requesting the antibody dilutions used to obtain the IHC (P) results for PB9475. Could you help provide that information? https://www.bosterbio.com/anti-aquaporin-4-picoband-trade-antibody-pb9475-boster.html?options=cart
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
The concentration of PB9457 used to generate IHC(P) image is 1ug/mL. Please reconstitute the antibody first as described below. Reconstitution: 0.2ml of distilled water will yield a concentration of 500?g/ml. Then dilute reconstituted antibody of 500ug/mL into 1ug/mL.
Boster Scientific Support
Answered: 2019-09-19
Question
I see that the anti-Aquaporin 4/AQP4 antibody PB9475 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-01
Question
you antibody using your anti-Aquaporin 4/AQP4 antibody for cellular response to interferon-gamma studies. Has this antibody been tested with western blotting on rat brain tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-04-03
Answer
Thank you for your inquiry. This PB9475 anti-Aquaporin 4/AQP4 antibody is validated on rat brain tissue, tissue lysate, mouse brain, human glioma tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-04-03
Question
We were happy with the WB result of your anti-Aquaporin 4/AQP4 antibody. However we have seen positive staining in lateral globus pallidus cell membrane using this antibody. Is that expected? Could you tell me where is AQP4 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-01-22
Answer
From what I have seen in literature, lateral globus pallidus does express AQP4. Generally AQP4 expresses in cell membrane. Regarding which tissues have AQP4 expression, here are a few articles citing expression in various tissues:
Fetal brain, Pubmed ID: 7559426
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-01-22
Question
I was wanting to use your anti-Aquaporin 4/AQP4 antibody for IHC for mouse lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse lung identification?
Verified Customer
Verified customer
Asked: 2017-09-07
Answer
It shows on the product datasheet, PB9475 anti-Aquaporin 4/AQP4 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-07
Question
Will anti-Aquaporin 4/AQP4 antibody PB9475 work for IHC with lung?
D. Mangal
Verified customer
Asked: 2016-12-23
Answer
According to the expression profile of lung, AQP4 is highly expressed in lung. So, it is likely that anti-Aquaporin 4/AQP4 antibody PB9475 will work for IHC with lung.
Boster Scientific Support
Answered: 2016-12-23
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-Aquaporin 4/AQP4 antibody PB9475. Let me know if you need anything else.
C. Anderson
Verified customer
Asked: 2015-02-19
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-02-19
Question
We are currently using anti-Aquaporin 4/AQP4 antibody PB9475 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
N. Jackson
Verified customer
Asked: 2013-03-01
Answer
The anti-Aquaporin 4/AQP4 antibody (PB9475) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-03-01
Question
See attached the WB image, lot number and protocol we used for lung using anti-Aquaporin 4/AQP4 antibody PB9475. Please let me know if you require anything else.
F. Carter
Verified customer
Asked: 2013-03-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-03-01
Question
My question regarding product PB9475, anti-Aquaporin 4/AQP4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Krishna
Verified customer
Asked: 2013-01-23
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9475 anti-Aquaporin 4/AQP4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-01-23
