Anti-Aquaporin 4/AQP4 Antibody Picoband™

Aquaporin-4 antibody

Boster Bio Anti-Aquaporin 4/AQP4 Antibody Picoband™ catalog # PB9475. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 9 publication(s).

Product Info Summary

SKU: PB9475
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Aquaporin 4/AQP4 Antibody Picoband™

View all Aquaporin-4 Antibodies

SKU/Catalog Number

PB9475

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Aquaporin 4/AQP4 Antibody Picoband™ catalog # PB9475. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Aquaporin 4/AQP4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9475)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 4 (295-323aa KPGVVHVIDVDRGEEKKGKDQSGEVLSSV), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB9475 is reactive to AQP4 in Human, Mouse, Rat

Applications

PB9475 is guaranteed for IHC, WB Boster Guarantee

Background of Aquaporin-4

Aquaporin 4 is found in the basolateral cell membrane of principal collecting duct cells and provides a pathway for water to exit these cells. The gene of AQP4 is mapped to 18q11.2-q12.1. Similar to other aquaporins, the AQP4 gene is composed of 4 exons encoding 127, 55, 27, and 92 amino acids separated by introns of 0.8, 0.3, and 5.2 kb. Unlike other aquaporins, an alternative coding initiation sequence (designated exon 0) was located 2.7 kb upstream of exon 1. When spliced together, M1 and the subsequent 10 amino acids are encoded by exon 0; the next 11 amino acids and M23 are encoded by exon 1. AQP4 is expressed in astrocytes and is upregulated by direct insult to the central nervous system. And AQP4 is the predominant water channel in the brain and has an important role in brain water homeostasis. It is abundant in mammalian brain and is concentrated in astrocytic foot processes at the blood-brain barrier.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human

Validation Images & Assay Conditions

Gene/Protein Information For AQP4 (Source: Uniprot.Org, NCBI)

Uniprot ID

P55087

Gene ID

361

Gene Name

AQP4

Full Name

Aquaporin-4

Weight

34.83kDa

Superfamily

MIP/aquaporin (TC 1.A.8) family

Alternative Names

aquaporin 4; MGC22454 AQP4 MIWC, WCH4 aquaporin 4 aquaporin-4|aquaporin type4|mercurial-insensitive water channel

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on AQP4, check out the AQP4 Infographic

AQP4 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for AQP4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PB9475 has been cited in 9 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Brain edema after intracerebral hemorrhage in rats: the role of iron overload and aquaporin 4: Laboratory investigation

Group 2 innate lymphoid cells suppress the pathology of neuromyelitis optica spectrum disorder

Expression of AQP1, AQP3, AQP4 and AQP5 in upper respiratory tract of buffaloes during different seasons

Song T,Chen W,Chen X,Zhang H,Zou Y,Wu H,Lin F,Ren L,Kang Y,Lei H.Repeated fluoxetine treatment induces transient and long-term astrocytic plasticity in the medial prefrontal cortex of normal adult rats.Prog Neuropsychopharmacol Biol Psychiatry.2021 Jan 20
Species: Rat
PB9475 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:3000

Changes in the Expression of AQP4 and AQP9 in the Hippocampus Following Eclampsia-Like Seizure

Expression pattern of aquaporins in patients with primary nephrotic syndrome with edema

Neuroprotective effects of total steroid saponins on cerebral ischemia injuries in animal model of focal ischemia/reperfusion

Anzabi M, Ardalan M, Iversen NK, Rafati AH, Hansen B, ?stergaard L. Front Cell Neurosci. 2018 Jan 31;12:19. doi: 10.3389/fncel.2018.00019. eCollection 2018. Hippocampal Atrophy Following Subarachnoid Hemorrhage Correlates with Disruption of Astroc...

Chen Hm, Huang Hs, Ruan L, He Yb, Li Xj. Int J Clin Exp Med. 2014 May 15;7(5):1483-9. Ecollection 2014. Ulinastatin Attenuates Cerebral Ischemia-Reperfusion Injury In Rats.

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18 Customer Q&As for Anti-Aquaporin 4/AQP4 Antibody Picoband™

Question

Do you have a BSA free version of anti-Aquaporin 4/AQP4 antibody PB9475 available?

Verified Customer

Verified customer

Asked: 2020-05-04

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Aquaporin 4/AQP4 antibody PB9475 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-05-04

Question

Would anti-Aquaporin 4/AQP4 antibody PB9475 work on primate IHC with lateral globus pallidus?

Verified Customer

Verified customer

Asked: 2020-02-20

Answer

Our lab technicians have not validated anti-Aquaporin 4/AQP4 antibody PB9475 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Aquaporin 4/AQP4 antibody PB9475 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate lateral globus pallidus in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-20

Question

I am interested in to test anti-Aquaporin 4/AQP4 antibody PB9475 on mouse lung for research purposes, then I may be interested in using anti-Aquaporin 4/AQP4 antibody PB9475 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-02-17

Answer

The products we sell, including anti-Aquaporin 4/AQP4 antibody PB9475, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-02-17

Question

We have been able to see staining in mouse lung. Do you have any suggestions? Is anti-Aquaporin 4/AQP4 antibody supposed to stain lung positively?

Verified Customer

Verified customer

Asked: 2020-01-17

Answer

Based on literature lung does express AQP4. Based on Uniprot.org, AQP4 is expressed in lateral globus pallidus, fetal brain, lung, among other tissues. Regarding which tissues have AQP4 expression, here are a few articles citing expression in various tissues:
Fetal brain, Pubmed ID: 7559426
Lung, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-01-17

Question

Would PB9475 anti-Aquaporin 4/AQP4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-01-16

Answer

It shows on the product datasheet, PB9475 anti-Aquaporin 4/AQP4 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-16

Question

Is this PB9475 anti-Aquaporin 4/AQP4 antibody reactive to the isotypes of AQP4?

Verified Customer

Verified customer

Asked: 2019-11-26

Answer

The immunogen of PB9475 anti-Aquaporin 4/AQP4 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 4 (295-323aa KPGVVHVIDVDRGEEKKGKDQSGEVLSSV), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-11-26

Question

We have tried in the past anti-Aquaporin 4/AQP4 antibody for WB on fetal brain a few months ago. I am using mouse, and We are going to use the antibody for IHC next. I am interested in examining fetal brain as well as lateral globus pallidus in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2019-11-15

Answer

I looked at the website and datasheets of our anti-Aquaporin 4/AQP4 antibody and it seems that PB9475 has been tested on mouse in both WB and IHC. Thus PB9475 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-11-15

Question

Is a blocking peptide available for product anti-Aquaporin 4/AQP4 antibody (PB9475)?

Verified Customer

Verified customer

Asked: 2019-11-05

Answer

We do provide the blocking peptide for product anti-Aquaporin 4/AQP4 antibody (PB9475). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-11-05

Question

A customer is requesting the antibody dilutions used to obtain the IHC (P) results for PB9475. Could you help provide that information? https://www.bosterbio.com/anti-aquaporin-4-picoband-trade-antibody-pb9475-boster.html?options=cart

Verified Customer

Verified customer

Asked: 2019-09-19

Answer

The concentration of PB9457 used to generate IHC(P) image is 1ug/mL. Please reconstitute the antibody first as described below. Reconstitution: 0.2ml of distilled water will yield a concentration of 500?g/ml. Then dilute reconstituted antibody of 500ug/mL into 1ug/mL.

Boster Scientific Support

Answered: 2019-09-19

Question

I see that the anti-Aquaporin 4/AQP4 antibody PB9475 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-07-01

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-07-01

Question

you antibody using your anti-Aquaporin 4/AQP4 antibody for cellular response to interferon-gamma studies. Has this antibody been tested with western blotting on rat brain tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-04-03

Answer

Thank you for your inquiry. This PB9475 anti-Aquaporin 4/AQP4 antibody is validated on rat brain tissue, tissue lysate, mouse brain, human glioma tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-04-03

Question

We were happy with the WB result of your anti-Aquaporin 4/AQP4 antibody. However we have seen positive staining in lateral globus pallidus cell membrane using this antibody. Is that expected? Could you tell me where is AQP4 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-01-22

Answer

From what I have seen in literature, lateral globus pallidus does express AQP4. Generally AQP4 expresses in cell membrane. Regarding which tissues have AQP4 expression, here are a few articles citing expression in various tissues:
Fetal brain, Pubmed ID: 7559426
Lung, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2018-01-22

Question

I was wanting to use your anti-Aquaporin 4/AQP4 antibody for IHC for mouse lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse lung identification?

Verified Customer

Verified customer

Asked: 2017-09-07

Answer

It shows on the product datasheet, PB9475 anti-Aquaporin 4/AQP4 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse lung in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-09-07

Question

Will anti-Aquaporin 4/AQP4 antibody PB9475 work for IHC with lung?

D. Mangal

Verified customer

Asked: 2016-12-23

Answer

According to the expression profile of lung, AQP4 is highly expressed in lung. So, it is likely that anti-Aquaporin 4/AQP4 antibody PB9475 will work for IHC with lung.

Boster Scientific Support

Answered: 2016-12-23

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-Aquaporin 4/AQP4 antibody PB9475. Let me know if you need anything else.

C. Anderson

Verified customer

Asked: 2015-02-19

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-02-19

Question

We are currently using anti-Aquaporin 4/AQP4 antibody PB9475 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?

N. Jackson

Verified customer

Asked: 2013-03-01

Answer

The anti-Aquaporin 4/AQP4 antibody (PB9475) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-03-01

Question

See attached the WB image, lot number and protocol we used for lung using anti-Aquaporin 4/AQP4 antibody PB9475. Please let me know if you require anything else.

F. Carter

Verified customer

Asked: 2013-03-01

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-03-01

Question

My question regarding product PB9475, anti-Aquaporin 4/AQP4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

M. Krishna

Verified customer

Asked: 2013-01-23

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9475 anti-Aquaporin 4/AQP4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2013-01-23

Size

Total: $315

SKU:PB9475

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

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