Anti-AR (N-terminal) Rabbit Monoclonal Antibody, Clone#RM254

Product Name

Anti-AR (N-terminal) Rabbit Monoclonal Antibody, Clone#RM254

View all Androgen R/NR3C4 Antibodies

SKU/Catalog Number

M00542-4

Size

100uL

Form

Liquid

Description

Boster Bio Anti-AR (N-terminal) Rabbit Monoclonal Antibody, Clone#RM254 (Catalog # M00542-4). Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-AR (N-terminal) Rabbit Monoclonal Antibody, Clone#RM254 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00542-4)

Host

Rabbit

Contents

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Clonality

Monoclonal

Clone Number

RM254

Isotype

Rabbit IgG

Immunogen

A peptide corresponding to the N-terminus of Androgen Receptor

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody reacts to full length androgen receptor and splice variants.

Reactive Species

M00542-4 is reactive to AR in Human

Applications

M00542-4 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

99.188kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (IHC): 1:1000 – 1:2500 dilution
WB: 1:100 – 1:1000 dilution.

Validation Images & Assay Conditions

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Figure 1. Western Blotting result
Western Blot analysis of 22RV1 whole cell lysates, using anti-Androgen Receptor (N-term) rabbit monoclonal antibody (Clone RM254), showed full length androgen receptor and splice variants expressed in 22RV1 cells.

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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human prostate cancer tissue section using anti-Androgen Receptor (N-term) rabbit monoclonal antibody (Clone RM254) at a 1:2500 dilution.

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Figure 3. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded 22RV1 cells sections using anti-Androgen Receptor (N-term) rabbit monoclonal antibody (Clone RM254) at a 1:2500 dilution.

Gene/Protein Information For AR (Source: Uniprot.org, NCBI)

Alternative Names

AIS; Androgen R; androgen receptor; AndrogenR; AR; DHTR; DHTRTFM; Dihydrotestosterone receptorHYSP1; HUMARA; NR3C4; NR3C4KD; Nuclear receptor subfamily 3 group C member 4; SBMA; SMAX1; SMAX1SBMA; spinal and bulbar muscular atrophy AR AIS8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM, AR androgen receptor androgen receptor|dihydrotestosterone receptor|nuclear receptor subfamily 3 group C member 4

For more info on AR, check out the AR Infographic

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In this infographic, you will see the following information for AR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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