Anti-ArfGAP3 Antibody

Product Name

Anti-ArfGAP3 Antibody

View all ARFGAP3 Antibodies

SKU/Catalog Number

A06839

Size

100uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-ArfGAP3 Antibody (Catalog # A06839). Tested in IF, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-ArfGAP3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06839)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Clone Number

3H11

Isotype

Antiserum

Immunogen

This whole rabbit serum was prepared by repeated immunizations with a truncated recombinant sequence of ArfGAP3 fused to GST.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A06839 is reactive to ARFGAP3 in Human

Applications

A06839 is guaranteed for IF, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

56.928kDa

Background of ARFGAP3

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. The ArfGAPs are a family of proteins encoded by 31 genes in humans. The function of the ArfGAPs is to regulate the small G protein Arf, a regulator of membrane traffic and actin cytoskeleton. The ArfGAPs have been found to be key regulators of cellular behaviors that involve coordinated actin and membrane remodeling, including protein secretion and migration. Several ArfGAPs are associated with cancer cell invasion and metastasis, and the Arf pathway has been found to be affected in a number of genetic diseases. Although the importance of the ArfGAPs and the Arf pathway for cellular physiology is well recognized, the molecular basis for the function of these proteins has not been established. Reagents for the studies, especially antibodies specific for particular members of the ArfGAP family that can be used for immunoblotting and immunofluorescence, are not available. ArfGAP3 is one of three ArfGAPs thought to be specifically associated with the Golgi apparatus; however, localization has only been done for ectopically expressed recombinant protein and cellular function is still not established. Indeed, there is a growing controversy about the function of ArfGAP3 together with ArfGAP1 and ArfGAP2. Antibodies suitable for immunoprecipitation, immunoblotting and immunofluorescence would be of great value in addressing the controversy and for discovering the potential role of ArfGAP3 in secretion of proteins, such as growth factors, and trafficking of other proteins to the cell surface, such as growth factor receptors and cell adhesion molecules.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5,000
IF Microscopy: 1:100
IP: User optimized
WB: 1:1,000

Validation Images & Assay Conditions

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Immunofluorescence Microscopy of Rabbit Anti-ArfGAP3 Antibody.Tissue:HeLa Whole Cell.Fixation:MeOH. Antigen retrieval:not required.Primary antibody:ArfGAP3 antibodyat 1:100 for 1 h at RT.Secondary antibody:Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT.Localization:ArfGAP3 is cytoplasmic.Staining:ArfGAP3 as green fluorescent sinal.

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Figure 2. Western blot analysis of ARFGAP3 using anti-ARFGAP3 antibody (A06839).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARFGAP3 antigen affinity purified polyclonal antibody (Catalog # A06839) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for ARFGAP3.

Gene/Protein Information For ARFGAP3 (Source: Uniprot.org, NCBI)

Alternative Names

ADP-ribosylation factor GTPase activating protein 1; ADP-ribosylation factor GTPase activating protein 3; ADP-ribosylation factor GTPase-activating protein 3; ARF GAP 3; ARFGAP1; FLJ45618 ARFGAP3 ARFGAP1 ADP ribosylation factor GTPase activating protein 3 ADP-ribosylation factor GTPase-activating protein 3|ADP-ribosylation factor GTPase activating protein 1|ARF GAP 3

For more info on ARFGAP3, check out the ARFGAP3 Infographic

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In this infographic, you will see the following information for ARFGAP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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