Anti-ARFGEF2 Antibody

ARFGEF2 antibody

Boster Bio Anti-ARFGEF2 Antibody (Catalog# A30581). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A30581
Size: 100ul
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IF, IHC-P, ICC, WB

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Product Name

Anti-ARFGEF2 Antibody

View all ARFGEF2 Antibodies

SKU/Catalog Number

A30581

Size

100ul

Form

Liquid

Description

Boster Bio Anti-ARFGEF2 Antibody (Catalog# A30581). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ARFGEF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30581)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A peptide derived from human ARFGEF2. Immunogen sequence location: 1491-1540

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A30581 is reactive to ARFGEF2 in Human, Mouse, Rat

Applications

A30581 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

202.038kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:20000
Not yet tested in other applications.

Validation Images & Assay Conditions

Gene/Protein Information For ARFGEF2 (Source: Uniprot.org, NCBI)

Gene Name

ARFGEF2

Full Name

Brefeldin A-inhibited guanine nucleotide-exchange protein 2

Weight

202.038kDa

Alternative Names

ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldinA-inhibited); ARFGEP2; BIG2brefeldin A-inhibited guanine nucleotide-exchange protein 2; Brefeldin A-inhibited GEP 2; dJ1164I10.1; FLJ23723; PVNH2 ARFGEF2 BIG2, PVNH2, dJ1164I10.1 ADP ribosylation factor guanine nucleotide exchange factor 2 brefeldin A-inhibited guanine nucleotide-exchange protein 2|ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)|brefeldin A-inhibited GEP 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ARFGEF2, check out the ARFGEF2 Infographic

ARFGEF2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ARFGEF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-ARFGEF2 Antibody

Question

Is there a BSA free version of anti-ARFGEF2 antibody A30581 available?

Verified Customer

Verified customer

Asked: 2020-01-13

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-ARFGEF2 antibody A30581 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-01-13

Question

My lab would like to test anti-ARFGEF2 antibody A30581 on mouse cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-ARFGEF2 antibody A30581 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-11-13

Answer

The products we sell, including anti-ARFGEF2 antibody A30581, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-11-13

Question

Will A30581 anti-ARFGEF2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-09-19

Answer

As indicated on the product datasheet, A30581 anti-ARFGEF2 antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-09-19

Question

See below the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-ARFGEF2 antibody A30581. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-07-03

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-07-03

Question

We are currently using anti-ARFGEF2 antibody A30581 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2018-04-20

Answer

The anti-ARFGEF2 antibody (A30581) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-04-20

Question

I have a question about product A30581, anti-ARFGEF2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

C. Carter

Verified customer

Asked: 2015-10-29

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30581 anti-ARFGEF2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2015-10-29

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-ARFGEF2 antibody A30581. Let me know if you need anything else.

M. Jones

Verified customer

Asked: 2013-05-23

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-05-23

Order DetailsPrice
A30581

100 μL

$335

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