Anti-Phospho-ARHGAP22 S397 Antibody

Product Name

Anti-Phospho-ARHGAP22 S397 Antibody

View all ARHGAP22 Antibodies

SKU/Catalog Number

P10197

Size

50ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Phospho-ARHGAP22 S397 Antibody (Catalog # P10197). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is six (6) months from date of opening.

Cite This Product

Anti-Phospho-ARHGAP22 S397 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P10197)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 50% (v/v) Glycerol

Clonality

Polyclonal

Isotype

IgG

Immunogen

ARHGAP22 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic phospho-peptide corresponding to the region surrounding mouse pS22 region of ARHGAP22.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P10197 is reactive to ARHGAP22 in Human, Mouse, Rat

Applications

P10197 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

76.779kDa

Background of ARHGAP22

ARHGAP22 is a Rho GTPase-activating protein involved in the signal transduction pathway that regulates endothelial cell capillary tube formation during angiogenesis. It acts as a GTPase activator for RAC1 by converting it to an inactive GDP-bound state and also inhibits RAC1-dependent lamellipodia formation. It may also play a role in transcription regulation via its interaction with VEZF1, by regulating activity of the endothelin-1 (EDN1) promoter. Anti-ARHGAP22 [p Ser22] antibody is ideal for researchers interested in Diabetes Research, Lipid and Metabolism research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:20,000 - 1:60,000
IHC: 1:100-1:500
IF Microscopy: 1:100-1:500
WB: 1 µg/ml

Validation Images & Assay Conditions

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Western blot analysis of ARHGAP22 expression in NIH3T3 cells transfected with a null vector (lane 1), NIH3T3 cells transfected with ARHGAP22 (lane 2), NIH3T3 cells transfected with ARHGAP22 S22 to alanine mutation (lane 3) and NIH3T3 cells transfected with ARHGAP22 S397 to alanine mutaion (lane 4). ARHGAP22 at 68KD was detected using rabbit anti-ARHGAP22 pS22 Antigen Affinity purified polyclonal antibody (left) and rabbit anti-ARHGAP22 pS397 Antigen Affinity purified polyclonal antibody (Catalog # P10197) (right) at 1 μg/mL.

Gene/Protein Information For ARHGAP22 (Source: Uniprot.org, NCBI)

Alternative Names

Rho GTPase activating protein 22; rho GTPase-activating protein 22; RhoGAP2; Rho-type GTPase-activating protein 22 ARHGAP22 RhoGAP2, RhoGap22 Rho GTPase activating protein 22 rho GTPase-activating protein 22|rho-type GTPase-activating protein 22

For more info on ARHGAP22, check out the ARHGAP22 Infographic

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In this infographic, you will see the following information for ARHGAP22: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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