Product Info Summary
SKU: | A05064 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-ARID2 Antibody Picoband™
SKU/Catalog Number
A05064
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ARID2 Antibody Picoband™ catalog # A05064. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ARID2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05064)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human ARID2, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05064 is reactive to Arid2 in Human, Mouse, Rat
Applications
A05064 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
200 kDa
Calculated molecular weight
195.988kDa
Background of Arid2
AT-rich interactive domain-containing protein 2 (ARID2) is a protein that in humans is encoded by the ARID2 gene. It is mapped to 12q12. This gene encodes a member of the AT-rich interactive domain (ARID)-containing family of DNA-binding proteins. Members of the ARID family have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation and chromatin structure modification. This protein functions as a subunit of the polybromo- and BRG1-associated factor or PBAF (SWI/SNF-B) chromatin remodeling complex which facilitates ligand-dependent transcriptional activation by nuclear receptors. Mutations in this gene are associated with hepatocellular carcinomas. A pseudogene of this gene is found on chromosome1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Validation Images & Assay Conditions
Click image to see more details
Figure 1. IHC analysis of ARID2 using anti-ARID2 antibody (A05064).
ARID2 was detected in paraffin-embedded section of mouse testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ARID2 Antibody (A05064) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 2. IHC analysis of ARID2 using anti-ARID2 antibody (A05064).
ARID2 was detected in paraffin-embedded section of human sarcoma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ARID2 Antibody (A05064) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of ARID2 using anti-ARID2 antibody (A05064).
ARID2 was detected in paraffin-embedded section of rat testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ARID2 Antibody (A05064) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. Western blot analysis of ARID2 using anti-ARID2 antibody (A05064).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human HL-60 whole cell lysates,
Lane 5: human A549 whole cell lysates,
Lane 6: rat testicular issue lysates,
Lane 7: mouse testicular issue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARID2 antigen affinity purified polyclonal antibody (Catalog # A05064) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARID2 at approximately 200KD. The expected band size for ARID2 is at 197KD.
Protein Target Info & Infographic
Gene/Protein Information For Arid2 (Source: Uniprot.org, NCBI)
Gene Name
Arid2
Full Name
AT-rich interactive domain-containing protein 2
Weight
195.988kDa
Superfamily
RFX family
Alternative Names
ARID domain-containing protein 2; AT rich interactive domain 2 (ARID, RFX-like); BAF200KIAA1557AT-rich interactive domain-containing protein 2; BRG1-associated factor 200; DKFZp686G052; DKFZp779P0222; FLJ30619; p200; Zinc finger protein with activation potential; zipzap; zipzap/p200 Arid2|1700124K17Rik, 4432409D24Rik, BAF200, zipzap/p200|AT rich interactive domain 2 (ARID, RFX-like)|AT-rich interactive domain-containing protein 2|ARID domain-containing protein 2|AT rich interactive domain 2 (Arid-rfx like)|BRG1-associated factor 200|zinc finger protein with activation potential|zipzap
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Arid2, check out the Arid2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Arid2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ARID2 Antibody Picoband™ (A05064)
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No publications found for A05064
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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10 Customer Q&As for Anti-ARID2 Antibody Picoband™
Question
Will anti-ARID2 antibody A05064 work on dog WB with kidney?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
Our lab technicians have not validated anti-ARID2 antibody A05064 on dog. You can run a BLAST between dog and the immunogen sequence of anti-ARID2 antibody A05064 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog kidney in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-17
Question
We are currently using anti-ARID2 antibody A05064 for human tissue, and we are happy with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
The anti-ARID2 antibody (A05064) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-05
Question
Would A05064 anti-ARID2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
You can see on the product datasheet, A05064 anti-ARID2 antibody as been tested on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-03
Question
Is this A05064 anti-ARID2 antibody reactive to the isotypes of ARID2?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
The immunogen of A05064 anti-ARID2 antibody is A synthetic peptide corresponding to a sequence of human ARID2 (DERRKGLAFLDELRQFHHSR). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-31
Question
Will anti-ARID2 antibody A05064 work for IHC-P with heart?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
According to the expression profile of heart, ARID2 is highly expressed in heart. So, it is likely that anti-ARID2 antibody A05064 will work for IHC-P with heart.
Boster Scientific Support
Answered: 2019-09-17
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for heart using anti-ARID2 antibody A05064. Let me know if you need anything else.
C. Gonzalez
Verified customer
Asked: 2019-07-23
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-23
Question
Is a blocking peptide available for product anti-ARID2 antibody (A05064)?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
We do provide the blocking peptide for product anti-ARID2 antibody (A05064). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-11
Question
you antibody to test anti-ARID2 antibody A05064 on mouse heart for research purposes, then I may be interested in using anti-ARID2 antibody A05064 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
The products we sell, including anti-ARID2 antibody A05064, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-16
Question
I see that the anti-ARID2 antibody A05064 works with IHC-P, what is the protocol used to produce the result images on the product page?
R. Li
Verified customer
Asked: 2018-10-17
Answer
You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-10-17
Question
Can you help my question with product A05064, anti-ARID2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Taylor
Verified customer
Asked: 2014-03-13
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05064 anti-ARID2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-03-13