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Product Info Summary
| SKU: | A03825 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-ARP Antibody
SKU/Catalog Number
A03825
Size
100μl
Form
Liquid
Description
Boster Bio Anti-ARP Antibody catalog # A03825. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ARP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03825)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human MANF. AA range:11-60
Reactive Species
A03825 is reactive to MANF in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
20.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03825 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC: 1:100-1:300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100
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Western Blot analysis of 293 cells using ARP Polyclonal Antibody. Secondary antibody was diluted at 1:20000
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Western blot analysis of lysate from 293 cells, using MANF Antibody.
Specific Publications For Anti-ARP Antibody (A03825)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-ARP Antibody
Question
We are currently using anti-ARP antibody A03825 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
The anti-ARP antibody (A03825) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-27
Question
Do you have a BSA free version of anti-ARP antibody A03825 available?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-ARP antibody A03825 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-13
Question
My lab would like to test anti-ARP antibody A03825 on mouse liver for research purposes, then I may be interested in using anti-ARP antibody A03825 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
The products we sell, including anti-ARP antibody A03825, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-24
Question
I was wanting to use your anti-ARP antibody for WB for mouse liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse liver identification?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
It shows on the product datasheet, A03825 anti-ARP antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-14
Question
Does anti-ARP antibody A03825 work on feline IHC with colon eye?
Verified Customer
Verified customer
Asked: 2019-04-18
Answer
Our lab technicians have not validated anti-ARP antibody A03825 on feline. You can run a BLAST between feline and the immunogen sequence of anti-ARP antibody A03825 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline colon eye in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-18
Question
I have attached the WB image, lot number and protocol we used for liver using anti-ARP antibody A03825. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-08-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-21
