Anti-Arrestin-C ARR3 Antibody

Product Name

Anti-Arrestin-C ARR3 Antibody

View all ARR3 Antibodies

SKU/Catalog Number

A06100

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Arrestin-C ARR3 Antibody (Catalog # A06100). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

ARR3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-Arrestin-C ARR3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06100)

Host

Rabbit

Contents

ARR3 antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

ARR3 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human ARR3. The immunogen is located within the last 50 amino acids of ARR3.

Cross-reactivity

ARR3 antibody is human, mouse and rat reactive. At least three isoforms of ARR3 are known to exist. This antibody is predicted to not cross-react with other members of the arrestin protein family.

Reactive Species

A06100 is reactive to ARR3 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

42.8 kDa

Background of ARR3

Arrestin 3 (ARR3) belongs to the Arrestin family of proteins that function as negative regulators of G protein-coupled receptor (GPCR) signaling (1,2). ARR3 binds the c-Jun N-terminal kinase 3 (JNK3) and scaffolds the apoptosis signal-regulating kinase 1 (ASK1)-MAPK kinase4-JNK3 cascade in a receptor-independent fashion, promoting JNK3 phosphorylation (3,4). ARR3 also mediates the internalization of the C-C chemokine receptor CCR7 upon the binding of its ligand CCL19, but not CCL21, suggesting that it helps mediate CCR7 receptor desensitization (5).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A06100 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ARR3 antibody can be used for detection of ARR3 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information ARR3

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