Anti-ARSI/Arylsulfatase I Antibody

Arsi antibody

Boster Bio Anti-ARSI/Arylsulfatase I Antibody (Catalog # A08580). Tested in WB, IF applications. This antibody reacts with Human.

Product Info Summary

SKU: A08580
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: IF, WB

Product Name

Anti-ARSI/Arylsulfatase I Antibody

View all Arsi Antibodies

SKU/Catalog Number

A08580

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-ARSI/Arylsulfatase I Antibody (Catalog # A08580). Tested in WB, IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ARSI/Arylsulfatase I Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08580)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human ARSI.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A08580 is reactive to Arsi in Human

Applications

A08580 is guaranteed for IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

64.424kDa

Background of Arsi

Displays arylsulfatase activity at neutral pH, when co-expressed with SUMF1; arylsulfatase activity is measured in the secretion medium of retinal cell line, but no activity is recorded when measured in cell extracts.

Sardiello M., Hum. Mol. Genet. 14:3203-3217(2005).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

Gene/Protein Information For Arsi (Source: Uniprot.org, NCBI)

Gene Name

Arsi

Full Name

Arylsulfatase I

Weight

64.424kDa

Superfamily

sulfatase family

Alternative Names

arylsulfatase family, member I; arylsulfatase I; ASI; EC 3.1.6; EC 3.1.6.-; EC 3.1.6.12; FLJ16069 Arsi|RGD1310242|arylsulfatase family, member I|arylsulfatase I|ASI

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Arsi, check out the Arsi Infographic

Arsi infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Arsi: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-ARSI/Arylsulfatase I Antibody

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-ARSI/Arylsulfatase I antibody A08580. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-04-21

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-21

Question

Would A08580 anti-ARSI/Arylsulfatase I antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

B. Zhao

Verified customer

Asked: 2017-08-18

Answer

You can see on the product datasheet, A08580 anti-ARSI/Arylsulfatase I antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-08-18

Question

See below the WB image, lot number and protocol we used for lung using anti-ARSI/Arylsulfatase I antibody A08580. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-07-05

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-07-05

Question

We are currently using anti-ARSI/Arylsulfatase I antibody A08580 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?

F. Jha

Verified customer

Asked: 2014-10-14

Answer

The anti-ARSI/Arylsulfatase I antibody (A08580) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-10-14

Order DetailsPrice
A08580-100uL

100uL

$360

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