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Product Info Summary
| SKU: | A06388 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Homeobox protein ARX ARX Antibody
SKU/Catalog Number
A06388
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Homeobox protein ARX ARX Antibody (Catalog # A06388). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Homeobox protein ARX ARX Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06388)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human ARX.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06388 is reactive to Arx in Human, Mouse, Rat
Applications
A06388 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
58.49kDa
Background of Arx
Transcription factor required for normal brain development. May be important for maintenance of specific neuronal subtypes in the cerebral cortex and axonal guidance in the floor plate.
Ohira R.H., Mol. Genet. Metab. 77:179-188(2002) [PubMed: 12359145].
Turner G., Am. J. Med. Genet. 112:405-411(2002) [PubMed: 12376946].
Bienvenu T., Hum. Mol. Genet. 11:981-991(2002) [PubMed: 11971879].
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from 293 cells, using ARX antibody A06388.
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ARX antibody A06388 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25
Protein Target Info & Infographic
Gene/Protein Information For Arx (Source: Uniprot.org, NCBI)
Gene Name
Arx
Full Name
Homeobox protein ARX
Weight
58.49kDa
Superfamily
paired homeobox family
Alternative Names
aristaless related homeobox; Aristaless-related homeobox; aristaless-related homeobox, X-linked; ARX; cancer/testis antigen 121; CT121; EIEE1; homeobox protein ARX; ISSX; ISSXmental retardation, X-linked 36; mental retardation, X-linked 29; mental retardation, X-linked 32; mental retardation, X-linked 33; mental retardation, X-linked 38; mental retardation, X-linked 43; mental retardation, X-linked 54; mental retardation, X-linked 76; mental retardation, X-linked 87; MRX29; MRX32; MRX33; MRX36; MRX38; MRX43; MRX54; MRX76; MRX87; MRXS1; PRTS Arx|Ar1, Arx|aristaless related homeobox|homeobox protein ARX
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Arx, check out the Arx Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Arx: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Homeobox protein ARX ARX Antibody (A06388)
Hello CJ!
No publications found for A06388
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Homeobox protein ARX ARX Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for female gonad using anti-ARX antibody A06388. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-10
Question
I was wanting to use your anti-ARX antibody for WB for mouse female gonad on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse female gonad identification?
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
It shows on the product datasheet, A06388 anti-ARX antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse female gonad in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-23
Question
Has A06388 been tested on mouse samples using IF?
Verified customer
Asked: 2019-07-16
Answer
Unfortunately, we have not tested the Anti-Homeobox Protein ARX ARX Antibody (A06388) in IF with mouse, so we're not sure. We suggest to run a pilot test if you want to try.
Boster Scientific Support
Answered: 2019-07-18
Question
Please see the WB image, lot number and protocol we used for female gonad using anti-ARX antibody A06388. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-02
Question
Would A06388 anti-ARX antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Williams
Verified customer
Asked: 2016-08-15
Answer
You can see on the product datasheet, A06388 anti-ARX antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-08-15
Question
Is this A06388 anti-ARX antibody reactive to the isotypes of ARX?
A. Walker
Verified customer
Asked: 2014-11-11
Answer
The immunogen of A06388 anti-ARX antibody is Synthesized peptide derived from internal of human ARX. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-11-11
Question
We are currently using anti-ARX antibody A06388 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
J. Edwards
Verified customer
Asked: 2013-06-12
Answer
The anti-ARX antibody (A06388) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-06-12
