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Product Info Summary
| SKU: | M06129-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-ASH2L Antibody (Center)
SKU/Catalog Number
M06129-1
Size
80 µl
Description
Boster Bio Anti-ASH2L Antibody (Center) (Catalog # M06129-1). Tested in WB application(s). This antibody reacts with Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ASH2L Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06129-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This ASH2L antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 347-382 amino acids from the Central region of human ASH2L.
Reactive Species
M06129-1 is reactive to ASH2L in Mouse
Calculated molecular weight
68.7 kDa
Background of ASH2L
Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06129-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
Validation Images & Assay Conditions
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All lanes : Anti-ASH2L Antibody (Center) at 1:2000 dilution
Lane 1: Hela whole cell lysates
Lane 2: Jurkat whole cell lysates
Lane 3: K562 whole cell lysates
Lane 4: F9 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 69 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-ASH2L Antibody (Center) at 1:2000 dilution + NIH/3T3 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 69 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-ASH2L Antibody (Center) (M06129-1)
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