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Product Info Summary
| SKU: | M04085 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Aspartate Aminotransferase Monoclonal Antibody
SKU/Catalog Number
M04085
BM5408 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Aspartate Aminotransferase Monoclonal Antibody catalog # M04085. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Aspartate Aminotransferase Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04085)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADEA-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Aspartate Aminotransferase Plays a key role in amino acid metabolism.
Reactive Species
M04085 is reactive to GOT1 in Human, Mouse, Rat
Observed Molecular Weight
41 kDa
Calculated molecular weight
46.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04085 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:20
Positive Control
WB: human MCF-7 whole cell, human K562 whole cell, human HepG2 whole cell, human A431 whole cell, rat heart tissue, rat liver tissue, mouse heart tissue, mouse liver tissue
Validation Images & Assay Conditions
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Western blot analysis of GOT1 using anti-GOT1 antibody (M04085).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human A431 whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GOT1 antigen affinity purified monoclonal antibody (M04085) at 1: 500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GOT1 at approximately 41 kDa. The expected band size for GOT1 is at 46 kDa.
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5 Customer Q&As for Anti-Aspartate Aminotransferase Monoclonal Antibody
Question
I am interested in to test anti-Aspartate Aminotransferase Monoclonal antibody M04085 on mouse brain for research purposes, then I may be interested in using anti-Aspartate Aminotransferase Monoclonal antibody M04085 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Carter
Verified customer
Asked: 2019-09-10
Answer
The products we sell, including anti-Aspartate Aminotransferase Monoclonal antibody M04085, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-10
Question
I was wanting to use your anti-Aspartate Aminotransferase Monoclonal antibody for IF for mouse brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse brain identification?
Verified Customer
Verified customer
Asked: 2018-01-25
Answer
As indicated on the product datasheet, M04085 anti-Aspartate Aminotransferase Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-25
Question
Does M04085 anti-Aspartate Aminotransferase Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-12-01
Answer
As indicated on the product datasheet, M04085 anti-Aspartate Aminotransferase Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-12-01
Question
Does anti-Aspartate Aminotransferase Monoclonal antibody M04085 work for IF with brain?
C. Gonzalez
Verified customer
Asked: 2013-10-16
Answer
According to the expression profile of brain, GOT1 is highly expressed in brain. So, it is likely that anti-Aspartate Aminotransferase Monoclonal antibody M04085 will work for IF with brain.
Boster Scientific Support
Answered: 2013-10-16
Question
We are currently using anti-Aspartate Aminotransferase Monoclonal antibody M04085 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
S. Krishna
Verified customer
Asked: 2013-03-05
Answer
The anti-Aspartate Aminotransferase Monoclonal antibody (M04085) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-03-05
