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Product Info Summary
| SKU: | A01099-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-ASXL1 Antibody Picoband™
SKU/Catalog Number
A01099-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ASXL1 Antibody Picoband™ catalog # A01099-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ASXL1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01099-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ASXL1 recombinant protein (Position: A14-R1541).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01099-1 is reactive to ASXL1 in Human
Applications
A01099-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
165 kDa
Calculated molecular weight
165.432kDa
Background of ASXL1
Putative Polycomb group protein ASXL1 is a protein that in humans is encoded by the ASXL1 gene. This gene is similar to the Drosophila additional sex combs gene, which encodes a chromatin-binding protein required for normal determination of segment identity in the developing embryo. The protein is a member of the Polycomb group of proteins, which are necessary for the maintenance of stable repression of homeotic and other loci. The protein is thought to disrupt chromatin in localized areas, enhancing transcription of certain genes while repressing the transcription of other genes. The protein encoded by this gene functions as a ligand-dependent co-activator for retinoic acid receptor in cooperation with nuclear receptor coactivator 1. Mutations in this gene are associated with myelodysplastic syndromes and chronic myelomonocytic leukemia. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ASXL1 using anti-ASXL1 antibody (A01099-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human COLO-32 whole cell lysates,
Lane 2: human Jurkat whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASXL1 antigen affinity purified polyclonal antibody (Catalog # A01099-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASXL1 at approximately 165KD. The expected band size for ASXL1 is at 165KD.
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Figure 2. Flow Cytometry analysis of SiHa cells using anti-ASXL1 antibody (A01099-1).
Overlay histogram showing SiHa cells stained with A01099-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ASXL1 Antibody (A01099-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For ASXL1 (Source: Uniprot.org, NCBI)
Gene Name
ASXL1
Full Name
Polycomb group protein ASXL1
Weight
165.432kDa
Superfamily
Asx family
Alternative Names
additional sex combs like 1 (Drosophila) ASXL1 BOPS, MDS ASXL transcriptional regulator 1 polycomb group protein ASXL1|additional sex combs like 1, transcriptional regulator|additional sex combs like transcriptional regulator 1|putative Polycomb group protein ASXL1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ASXL1, check out the ASXL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ASXL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ASXL1 Antibody Picoband™ (A01099-1)
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No publications found for A01099-1
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