Product Info Summary
SKU: | PB9422 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Ataxin 1/ATXN1 Antibody Picoband™
SKU/Catalog Number
PB9422
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Ataxin 1/ATXN1 Antibody Picoband™ catalog # PB9422. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ataxin 1/ATXN1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9422)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Ataxin 1, different from the related mouse and rat sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9422 is reactive to ATXN1 in Human, Mouse, Rat
Applications
PB9422 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
105 kDa
Calculated molecular weight
86.923kDa
Background of Ataxin 1
Ataxin-1 is a protein that in humans is encoded by the ATXN1 gene. The ATXN1 gene had been mapped to 6p23 by in situ hybridization. Ataxin-1 (ATXN1), a causative factor for spinocerebellar ataxia type 1 (SCA1), and the related Brother of ATXN1 (BOAT1) are human proteins involved in transcriptional repression. ATXN1 and BOAT1 might participate in several Notch-controlled developmental and pathological processes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Ataxin 1 using anti-Ataxin 1 antibody (PB9422).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ataxin 1 antigen affinity purified polyclonal antibody (Catalog # PB9422) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ataxin 1 at approximately 105 kDa. The expected band size for Ataxin 1 is at 105 kDa.
Click image to see more details
Figure 2. IHC analysis of Ataxin 1 using anti-Ataxin 1 antibody (PB9422).
Ataxin 1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ataxin 1 Antibody (PB9422) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Ataxin 1 using anti-Ataxin 1 antibody (PB9422).
Ataxin 1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ataxin 1 Antibody (PB9422) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Ataxin 1 using anti-Ataxin 1 antibody (PB9422).
Ataxin 1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ataxin 1 Antibody (PB9422) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 5. IF analysis of Ataxin 1 using anti-Ataxin 1 antibody (PB9422).
Ataxin 1 was detected in immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- Ataxin 1 Antibody (PB9422) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 6. Flow Cytometry analysis of SiHa cells using anti-Ataxin 1 antibody (PB9422).
Overlay histogram showing SiHa cells stained with PB9422 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Ataxin 1 Antibody (PB9422, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For ATXN1 (Source: Uniprot.org, NCBI)
Gene Name
ATXN1
Full Name
Ataxin-1
Weight
86.923kDa
Superfamily
ATXN1 family
Alternative Names
ataxin 1; ataxin 1); ataxin-1; ATX1spinocerebellar ataxia 1 (olivopontocerebellar ataxia 1, autosomal dominant; SCA1D6S504E; Spinocerebellar ataxia type 1 protein ATXN1 ATX1, D6S504E, SCA1 ataxin 1 ataxin-1|alternative ataxin1|spinocerebellar ataxia type 1 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATXN1, check out the ATXN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATXN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ataxin 1/ATXN1 Antibody Picoband™ (PB9422)
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No publications found for PB9422
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Customer Q&As
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4 Customer Q&As for Anti-Ataxin 1/ATXN1 Antibody Picoband™
Question
Can PB9422 work on mouse samples?
Verified customer
Asked: 2019-04-25
Answer
Our lab test results on mouse samples are not good, so we do not recommend using the Anti-Ataxin 1/ATXN1 Antibody Picoband™ (PB9422) on mouse.
Boster Scientific Support
Answered: 2019-04-26
Question
Would PB9422 anti-Ataxin 1/ATXN1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Carter
Verified customer
Asked: 2018-08-06
Answer
It shows on the product datasheet, PB9422 anti-Ataxin 1/ATXN1 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-08-06
Question
I see that the anti-Ataxin 1/ATXN1 antibody PB9422 works with IHC, what is the protocol used to produce the result images on the product page?
S. Patel
Verified customer
Asked: 2014-10-29
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-10-29
Question
We are currently using anti-Ataxin 1/ATXN1 antibody PB9422 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
G. Jha
Verified customer
Asked: 2013-03-06
Answer
The anti-Ataxin 1/ATXN1 antibody (PB9422) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-03-06