Product Info Summary
SKU: | M00916 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ATF2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00916
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ATF2 Rabbit Monoclonal Antibody catalog # M00916. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ATF2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00916)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IA-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ATF2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00916 is reactive to ATF2 in Human
Applications
M00916 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
48 kDa
Calculated molecular weight
54.537kDa
Background of ATF2
Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:100
ICC/IF 1:50-1:100
IP 1:30
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of ATF2 expression in HeLa cell lysate.
Click image to see more details
Immunofluorescent analysis of Hela cells, using ATF2 Antibody .
Protein Target Info & Infographic
Gene/Protein Information For ATF2 (Source: Uniprot.org, NCBI)
Gene Name
ATF2
Full Name
Cyclic AMP-dependent transcription factor ATF-2
Weight
54.537kDa
Superfamily
bZIP family
Alternative Names
activating transcription factor 2formerly; ATF2; cAMP responsive element binding protein 2; cAMP-responsive element-binding protein 2; CREB2; CREBP1; CRE-BP1; cyclic AMP-dependent transcription factor ATF-2; HB16; MGC111558; mXBP; TREB7 ATF2 CRE-BP1, CREB-2, CREB2, HB16, TREB7 activating transcription factor 2 cyclic AMP-dependent transcription factor ATF-2|activating transcription factor 2 splice variant ATF2-var2|cAMP response element-binding protein CRE-BP1|cAMP responsive element binding protein 2, formerly|cAMP-dependent transcription factor ATF-2|cyclic AMP-responsive element-binding protein 2|histone acetyltransferase ATF2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATF2, check out the ATF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATF2 Rabbit Monoclonal Antibody (M00916)
Hello CJ!
No publications found for M00916
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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5 Customer Q&As for Anti-ATF2 Rabbit Monoclonal Antibody
Question
See below the WB image, lot number and protocol we used for liver using anti-ATF2 Rabbit Monoclonal antibody M00916. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-18
Question
I see that the anti-ATF2 Rabbit Monoclonal antibody M00916 works with IP, what is the protocol used to produce the result images on the product page?
M. Collins
Verified customer
Asked: 2018-04-03
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-04-03
Question
Is there a BSA free version of anti-ATF2 Rabbit Monoclonal antibody M00916 available?
J. Carter
Verified customer
Asked: 2018-01-15
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ATF2 Rabbit Monoclonal antibody M00916 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-15
Question
Does anti-ATF2 Rabbit Monoclonal antibody M00916 work for IP with liver?
M. Williams
Verified customer
Asked: 2017-10-06
Answer
According to the expression profile of liver, ATF2 is highly expressed in liver. So, it is likely that anti-ATF2 Rabbit Monoclonal antibody M00916 will work for IP with liver.
Boster Scientific Support
Answered: 2017-10-06
Question
I was wanting to use your anti-ATF2 Rabbit Monoclonal antibody for IP for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
N. Dhar
Verified customer
Asked: 2015-08-21
Answer
It shows on the product datasheet, M00916 anti-ATF2 Rabbit Monoclonal antibody has been validated for IP, IF, IHC, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-08-21