Anti-ATF6 Antibody

Product Name

Anti-ATF6 Antibody

View all ATF6 Antibodies

SKU/Catalog Number

A00655-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-ATF6 Antibody (Catalog # A00655-1). Tested in ELISA, WB, IHC-P, ICC, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

ATF6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-ATF6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00655-1)

Host

Rabbit

Contents

ATF6 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

ATF6 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ATF6. The immunogen is located within amino acids 470 - 520 of ATF6.

Reactive Species

A00655-1 is reactive to ATF6 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

74.6 kDa

Background of ATF6

Disruptions of protein folding and maturation in the endoplasmic reticulum (ER) result in the activation of the unfolded protein response (UPR), an integrated cellular signaling pathway that transmits information from the ER lumen to the cytoplasm and nucleus. Activating transcription factor 6 (ATF6) as well as the ER-transmembrane protein kinases IRE1p and PERK are the major transducers of the UPR. ATF6 is an ER transmembrane protein that is normally bound to the ER chaperone GRP78, but upon ER stress is released from GRP78 and proteolytically cleaved to yield a cytosolic fragment which then migrates to the nucleus, and together with the transcription factor XBP-1, activates transcription of UPR-responsive genes. ATF6 has two isoforms (ATF6α and ATF6β); only ATF6α is recognized by this antibody.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00655-1 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ATF6 antibody can be used for the detection of ATF6 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL and immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information ATF6

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