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Product Info Summary
| SKU: | A07803 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-ATG10 Antibody
SKU/Catalog Number
A07803
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ATG10 Antibody (Catalog # A07803). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ATG10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ATG10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07803)
Host
Rabbit
Contents
ATG10 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ATG10 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human ATG10. The immunogen is located within the last 50 amino acids of ATG10.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
AJAP1 antibody is human, mouse and rat reactive.
Reactive Species
A07803 is reactive to Atg10 in Human, Mouse, Rat
Applications
A07803 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
24.577kDa
Background of Atg10
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy protein family is ATG10, an E2-like enzyme involved in two ubiquitin-like modifications essential for autophagosome formation: ATG12-ATG5 conjugation and the modification of a soluble form of MAP-LC3, a homolog of yeast Apg8, to a membrane-bound form. ATG10 has also been shown to interact with ATG12 in human embryonic kidney cells in the presence of ATG7. Multiple isoforms of ATG10 are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ATG10 antibody can be used for the detection of ATG10 by Western blot at 0.5 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ATG10 in SK-N-SH cell lysate with ATG10 antibody at (A) 0.5
Protein Target Info & Infographic
Gene/Protein Information For Atg10 (Source: Uniprot.org, NCBI)
Gene Name
Atg10
Full Name
Ubiquitin-like-conjugating enzyme ATG10
Weight
24.577kDa
Superfamily
ATG10 family
Alternative Names
APG10 autophagy 10-like (S. cerevisiae); APG10; APG10L; APG10-like; ATG10 autophagy related 10 homolog (S. cerevisiae); ATG10; Autophagy-related protein 10; DKFZp586I0418; EC 6.3.2.-; FLJ13954; PP12616; ubiquitin-like-conjugating enzyme ATG10
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Atg10, check out the Atg10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Atg10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATG10 Antibody (A07803)
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No publications found for A07803
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Customer Reviews
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