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Product Info Summary
| SKU: | A11053 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-ATG2B Antibody
SKU/Catalog Number
A11053
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ATG2B Antibody (Catalog # A11053). Tested in ELISA, WB, IF applications. This antibody reacts with Human.
Storage & Handling
ATG2B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ATG2B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11053)
Host
Rabbit
Contents
ATG2B Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ATG2B antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human ATG2B. The immunogen is located within amino acids 450 - 500 of ATG2B.
Cross-reactivity
ATG2B antibody is predicted to not cross-react with other ATG2A.
Reactive Species
A11053 is reactive to ATG2B in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
232.8 kDa
Background of ATG2B
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy family of proteins is ATG2B, one of two homologs of ATG2 that is essential for autophagosome formation and important for regulation of size and distribution of lipid droplets. Relatively high rates of ATG2B mutations were observed in gastric and colorectal carcinomas, suggesting that deregulating the autophagy process may contribute to cancer development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11053 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ATG2B antibody can be used for detection of ATG2B by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ATG2B in K562 cell lysate with ATG2B antibody at (A) 1 and (B) 2 μg/ml
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Immunofluorescence of ATG2B in K562 cells with ATG2B antibody at 20 μg/mL.
Specific Publications For Anti-ATG2B Antibody (A11053)
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