Click image to see more details
Product Info Summary
| SKU: | M09793 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IF, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-ATG4D Antibody
SKU/Catalog Number
M09793
Size
80 µl
Description
Boster Bio Anti-ATG4D Antibody (Catalog # M09793). Tested in IF, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ATG4D Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M09793)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
222CT15.4.1
Isotype
IgG1,K
Immunogen
This ATG4D monoclonal antibody is generated from mouse immunized with ATG4D recombinant protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M09793 is reactive to ATG4D in Human
Applications
M09793 is guaranteed for IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
52.922kDa
Background of ATG4D
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:4000
Validation Images & Assay Conditions
Click image to see more details
Immunofluorescent analysis of U251 cells, using ATG4D Antibody. U251 cells (right) were treated with Chloroquine (50 μM,16h). M09793 was diluted at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).
Click image to see more details
Immunofluorescent analysis of U251 cells
Click image to see more details
Anti-ATG4D at 1:4000 dilution + A431 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 53 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Protein Target Info & Infographic
Gene/Protein Information For ATG4D (Source: Uniprot.org, NCBI)
Gene Name
ATG4D
Full Name
Cysteine protease ATG4D
Weight
52.922kDa
Superfamily
peptidase C54 family
Alternative Names
APG4 autophagy 4 homolog D (S. cerevisiae); APG4 autophagy 4 homolog D; APG4D; APG4-D; ATG4 autophagy related 4 homolog D (S. cerevisiae); AUTL4; AUT-like 4 cysteine endopeptidase; AUT-like 4, cysteine endopeptidase (S. cerevisiae); AUT-like 4, cysteine endopeptidase; autophagin-4; Autophagy-related cysteine endopeptidase 4; Autophagy-related protein 4 homolog D; cysteine protease ATG4D; cysteine protease involved in autophagy; EC 3.4.22; EC 3.4.22.- ATG4D APG4-D, APG4D, AUTL4 autophagy related 4D cysteine peptidase cysteine protease ATG4D|APG4 autophagy 4 homolog D|ATG4 autophagy related 4 homolog D|AUT-like 4 cysteine endopeptidase|autophagin-4|autophagy-related cysteine endopeptidase 4|autophagy-related protein 4 homolog D|cysteine protease involved in autophagy
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATG4D, check out the ATG4D Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATG4D: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATG4D Antibody (M09793)
Hello CJ!
No publications found for M09793
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-ATG4D Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-ATG4D Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
