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Product Info Summary
| SKU: | M09793 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IF, WB |
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Product info
Product Name
Anti-ATG4D Antibody
SKU/Catalog Number
M09793
Size
80 µl
Description
Boster Bio Anti-ATG4D Antibody (Catalog # M09793). Tested in IF, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ATG4D Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M09793)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
222CT15.4.1
Isotype
IgG1,K
Immunogen
This ATG4D monoclonal antibody is generated from mouse immunized with ATG4D recombinant protein.
Reactive Species
M09793 is reactive to ATG4D in Human
Calculated molecular weight
52.9 kDa
Background of ATG4D
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M09793 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:4000
Validation Images & Assay Conditions
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Immunofluorescent analysis of U251 cells, using ATG4D Antibody. U251 cells (right) were treated with Chloroquine (50 μM,16h). M09793 was diluted at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).
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Immunofluorescent analysis of U251 cells
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Anti-ATG4D at 1:4000 dilution + A431 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 53 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-ATG4D Antibody (M09793)
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