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- Table of Contents
ATG5 antibody (M00240) performs well in IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 14 Publications
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Immunofluorescent analysis of Raji cells, using ATG5 Antibody.
Designing a Western blot or IHC for ATG5? The guides cover the expected band size and staining pattern, antibodies backed by real images, the controls to run, and protocols from published papers.
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Anti-ATG5/Apg5 Rabbit Monoclonal Antibody
ATG5 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IP, IF, IHC, ICC, WB |
Tested Applications
ATG5 antibody was positively detected by WB in:
human Hela whole cell, human 293T whole cell, human MCF-7 whole cell, human K562 whole cell, rat liver tissue, rat C6 whole cell, mouse liver tissue, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ATG5 antibody was positively detected by IHC in:
human ovarian cancer tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
ATG5 antibody was positively detected by ICC/IF in:
Raji cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
IP
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Product Information
Product Name
Anti-ATG5/Apg5 Rabbit Monoclonal Antibody
View all ATG5 Antibodies
View all ATG5 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
GCD-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ATG5
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
55 kDa
Calculated Molecular Weight
32.4 kDa
Cite This Product
Anti-ATG5/Apg5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00240)
Protocols
Western Blot protocol for ATG5 antibody
Immunohistochemistry (IHC) protocol for ATG5 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for ATG5 antibody
Publications
Application:
Species:
TRPV1 mediates oxidative stress and autophagy pathways to regulate airway MUC5AC secretion
Published: 2025-12-17
Journal: BIOCHEMICAL PHARMACOLOGY
Application: WB
Impact Factor: 5.60
Species: Human,Mouse
PMID: 41418942
Hydrogen alleviates cardiac ferroptosis damage under chronic intermittent hypoxia conditions by targeting Nrf2
Published: 2025-12-09
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: WB
Impact Factor: 4.70
Species: Mouse
PMID: 41370977
Momordica Charantia L. polysaccharides ameliorate colonic mucus barrier damage induced by high fat diet through regulation of gut microbiota and MUC2 expression
Published: 2025-10-03
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Application: WB
Impact Factor: 8.50
Species: Mouse
PMID: 41046890
R406 and its structural analogs reduce SNCA/α-synuclein levels via autophagic degradation
Published: 2025-04-04
Journal: Autophagy
Application: WB
Impact Factor: 14.30
Species: Rat
PMID: 40143425
NCX1/Ca2+ promotes autophagy and decreases bortezomib activity in multiple myeloma through non-canonical NFκB signaling pathway
Published: 2024-05-06
Journal: Cell Communication and Signaling
Application: WB
Impact Factor: 8.20
Species: Human
PMID: 38711131
Liensinine sensitizes colorectal cancer cells to oxaliplatin by targeting HIF-1a to inhibit autophagy
Published: 2024-04-22
Journal: PHYTOMEDICINE
Application: IHC
Impact Factor: 6.70
Species: Mouse
PMID: 38703660
1,3-Substituted β-Carboline Derivatives as Potent Chemotherapy for the Treatment of Cystic Echinococcosis
Published: 2023-12-09
Journal: JOURNAL OF MEDICINAL CHEMISTRY
Application: WB
Impact Factor: 7.30
Species: Sheep
PMID: 38069814
Synergistic Lysosomal Impairment and ER Stress Activation for Boosted Autophagy Dysfunction Based on Te Double-Headed Nano-Bullets
Published: 2022-05-29
Journal: Small
Application: WB
Impact Factor: 15.15
Species: Mouse
PMID: 35644863
DJ-1 activates the Atg5-Atg12-Atg16L1 complex via Sirt1 to influence microglial polarization and alleviate cerebral ischemia/reperfusion-induced inflammatory injury
Published: 2022-04-14
Journal: NEUROCHEMISTRY INTERNATIONAL
Application: IP,WB
Impact Factor: 4.30
Species: Rat
PMID: 35429577
Integrated metabolomics and mechanism to reveal the protective effect of kaempferol on pulmonary arterial hypertension
Published: 2022-02-15
Journal: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Application: WB
Impact Factor: 3.57
Species: Rat
PMID: 35202944
eIF3k inhibits NF-κB signaling by targeting MyD88 for ATG5-mediated autophagic degradation in teleost fish
Published: 2022-02-14
Journal: JOURNAL OF BIOLOGICAL CHEMISTRY
Application: WB,IF
Impact Factor: 5.49
Species: Fish
PMID: 35176284
Dependence of glucose transport on autophagy and GAPDH activity
Published: 2021-12-02
Journal: BRAIN RESEARCH
Application: WB
Impact Factor: 3.25
Species: Human
PMID: 34864044
Cardiac Glycoside Compound Isolated from Helleborus thibetanus Franch Displays Potent Toxicity against HeLa Cervical Carcinoma Cells through ROS-Independent Autophagy
Published: 2019-11-12
Journal: CHEMICAL RESEARCH IN TOXICOLOGY
Application: WB
Impact Factor: 3.27
Species: Human
PMID: 31714069
Ginsenoside Rb3 provides protective effects against cisplatin-induced nephrotoxicity via regulation of AMPK-/mTOR-mediated autophagy and inhibition of apoptosis in vitro and in vivo
Published: 2019-05-16
Journal: CELL PROLIFERATION
Application: IF,WB
Impact Factor: 5.04
Species: Mouse
PMID: 31094028
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Product Promise
We promise Anti-ATG5/Apg5 Rabbit Monoclonal Antibody performs excellently in relevant applications for IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M00240
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 23, 2026