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Product Info Summary
| SKU: | A09625 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-ATP11A Antibody
SKU/Catalog Number
A09625
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ATP11A Antibody (Catalog # A09625). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ATP11A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ATP11A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09625)
Host
Rabbit
Contents
ATP11A Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
ATP11A antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human ATP11A. The immunogen is located within the first 50 amino acids of ATP11A.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
VEZT antibody is human, mouse and rat reactive. Multiple isoforms are known to exist.
Reactive Species
A09625 is reactive to ATP11A in Human, Mouse, Rat
Applications
A09625 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
129.756kDa
Background of ATP11A
ATP11A is a widely expressed integral membrane ATPase. ATP11A is probably phosphorylated in its intermediate state and likely drives the transport of ions such as calcium or other molecules across membranes. While the exact molecule ATP11A transports is unknown, increased expression of ATP11A mRNA has been observed in murine leukemia cells made resistant to anti-cancer drugs such as farnesyltransferase inhibitors (FTIs). Furthermore, overexpression of ATP11A provided protection against the FTI SCH66336 while knockdown of ATP11A via siRNA made cells more sensitive to this drug. Other reports suggest that elevated levels of ATP11A mRNA may also be a predictive marker of metastasis in colorectal cancer patients.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ATP11A antibody can be used for detection of ATP11A by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ATP11A in K562 cell tissue lysate with ATP11A antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunocytochemistry of ATP11A in K562 cells with ATP11A antibody at 10 μg/mL.
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Immunofluorescence of ATP11A in K562 cells with ATP11A antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For ATP11A (Source: Uniprot.org, NCBI)
Gene Name
ATP11A
Full Name
Probable phospholipid-transporting ATPase IH
Weight
129.756kDa
Superfamily
cation transport ATPase (P-type) (TC 3.A.3) family
Alternative Names
ATPase, class VI, type 11A ATP11A ATPIH, ATPIS ATPase phospholipid transporting 11A probable phospholipid-transporting ATPase IH|ATPase, class VI, type 11A|P4-ATPase flippase complex alpha subunit ATP11A|phospholipid-translocating ATPase|potential phospholipid-transporting ATPase IH
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATP11A, check out the ATP11A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATP11A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATP11A Antibody (A09625)
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