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Product Info Summary
| SKU: | A07149 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-ATP1AL1 ATP12A Antibody
SKU/Catalog Number
A07149
Size
100μl
Form
Liquid
Description
Boster Bio Anti-ATP1AL1 ATP12A Antibody catalog # A07149. Tested in WB, ELISA applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ATP1AL1 ATP12A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07149)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from ATP1AL1 . at AA range: 380-460
Reactive Species
A07149 is reactive to ATP12A in Human, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
115.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07149 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of extracts from rat stomach, using ATP1AL1 Polyclonal Antibody. Secondary antibody was diluted at 1:20000
Specific Publications For Anti-ATP1AL1 ATP12A Antibody (A07149)
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6 Customer Q&As for Anti-ATP1AL1 ATP12A Antibody
Question
We are currently using anti-ATP1AL1 antibody A07149 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
The anti-ATP1AL1 antibody (A07149) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-24
Question
Is a blocking peptide available for product anti-ATP1AL1 antibody (A07149)?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
We do provide the blocking peptide for product anti-ATP1AL1 antibody (A07149). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-02-21
Question
We need to test anti-ATP1AL1 antibody A07149 on human kidney skin for research purposes, then I may be interested in using anti-ATP1AL1 antibody A07149 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
The products we sell, including anti-ATP1AL1 antibody A07149, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-22
Question
Is this A07149 anti-ATP1AL1 antibody reactive to the isotypes of ATP12A?
Verified Customer
Verified customer
Asked: 2019-07-17
Answer
The immunogen of A07149 anti-ATP1AL1 antibody is Synthesized peptide derived from the Internal region of human ATP1AL1. at AA rangle: 380-460. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-17
Question
Does anti-ATP1AL1 antibody A07149 work on bovine WB with liver?
G. Kulkarni
Verified customer
Asked: 2018-09-03
Answer
Our lab technicians have not tested anti-ATP1AL1 antibody A07149 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-ATP1AL1 antibody A07149 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-03
Question
I have a question about product A07149, anti-ATP1AL1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
C. Anderson
Verified customer
Asked: 2016-07-27
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07149 anti-ATP1AL1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-07-27
