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Product Info Summary
| SKU: | A07027-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-ATP1B2 Antibody (Center)
SKU/Catalog Number
A07027-1
Size
80 µl
Description
Boster Bio Anti-ATP1B2 Antibody (Center) (Catalog # A07027-1). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ATP1B2 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07027-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This ATP1B2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-141 amino acids from the Central region of human ATP1B2.
Reactive Species
A07027-1 is reactive to ATP1B2 in Human
Calculated molecular weight
33.4 kDa
Background of ATP1B2
The protein belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07027-1 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:50-1:100
FC: 1:10-1:50
Validation Images & Assay Conditions
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Western blot analysis of lysates from K562 cell line and rat liver tissue lysate (from left to right), using ATP1B2 Antibody (Center). A07027-1 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ATP1B2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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ATP1B2 Antibody (Center) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Specific Publications For Anti-ATP1B2 Antibody (Center) (A07027-1)
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