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Product Info Summary
| SKU: | M04597 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-ATP2A3 Antibody (N-Term)
SKU/Catalog Number
M04597
Size
50 µl
Description
Boster Bio Anti-ATP2A3 Antibody (N-Term) (Catalog # M04597). Tested in WB, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ATP2A3 Antibody (N-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04597)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This ATP2A3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 13-47 amino acids from human ATP2A3.
Reactive Species
M04597 is reactive to ATP2A3 in Human
Calculated molecular weight
114.0 kDa
Background of ATP2A3
This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04597 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-ATP2A3 Antibody (N-Term) at 1:2000 dilution
Lane 1: HL-60 whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: K562 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 114 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M04597 staining ATP2A3 in human brain tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Specific Publications For Anti-ATP2A3 Antibody (N-Term) (M04597)
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