Anti-ATP2C1 Antibody

Product Name

Anti-ATP2C1 Antibody

View all ATP2C1 Antibodies

SKU/Catalog Number

A03143

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-ATP2C1 Antibody (Catalog # A03143). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

ATP2C1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-ATP2C1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03143)

Host

Rabbit

Contents

ATP2C1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

Clone: SK7

Isotype

IgG

Immunogen

ATP2C1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human ATP2C1. The immunogen is located within the last 50 amino acids of ATP2C1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least four isoforms of ATP2C1 are known to exist; this antibody will recognize only the three longest isoforms. ATP2C1 antibody will not cross-react with ATP2C2.

Reactive Species

A03143 is reactive to ATP2C1 in Human, Mouse

Applications

A03143 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

100.577kDa

Background of ATP2C1

ATP2C1, also known as secretory pathway Ca2+/Mn2+-ATPase (SPCA) 1, belongs to the family of P-type cation transport ATPases. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium from the cytosol to the Golgi lumen. Defects in this gene cause Hailey-Hailey disease, an autosomal dominant disorder characterized by persistent blisters and erosions of the skin. Unlike the related protein ATP2C2, ATP2C1 is ubiquitously expressed and displays a lower maximal turnover rate for overall Ca2+-ATPase reaction and a higher apparent affinity for cytosolic Ca2+ activation of phosphorylation. Recent evidence suggests that ATP2C1 is a key regulator of insulin-like growth factor receptor (IGF1R) processing in tumor progression in basal breast cancers.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ATP2C1 antibody can be used for detection of ATP2C1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of ATP2C1 in mouse brain tissue lysate with ATP2C1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.

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Immunohistochemistry of ATP2C1 in mouse brain tissue with ATP2C1 antibody at 5 μg/mL.

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Immunofluorescence of ATP2C1 in mouse brain tissue with ATP2C1 antibody at 20 μg/mL.

Gene/Protein Information For ATP2C1 (Source: Uniprot.org, NCBI)

Alternative Names

ATPase, Ca++ transporting, type 2C, member 1; BCPM; benign chronic pemphigus (Hailey-Hailey disease); Ca(2+)-sequestering; calcium-transporting ATPase type 2C member 1; EC 3.6.3; HHD; hSPCA1; HUSSY-28; PMR1L; secretory pathway Ca2+/Mn2+ ATPase 1 ATP2C1 ATP2C1A, BCPM, HHD, PMR1, SPCA1, hSPCA1 ATPase secretory pathway Ca2+ transporting 1 calcium-transporting ATPase type 2C member 1|ATP-dependent Ca(2+) pump PMR1|ATPase 2C1|ATPase, Ca(2+)-sequestering|ATPase, Ca++ transporting, type 2C, member 1|HUSSY-28|secretory pathway Ca2+/Mn2+ ATPase 1

For more info on ATP2C1, check out the ATP2C1 Infographic

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In this infographic, you will see the following information for ATP2C1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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