Anti-ATP2C2 Antibody

Product Name

Anti-ATP2C2 Antibody

View all ATP2C2 Antibodies

SKU/Catalog Number

A09207

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-ATP2C2 Antibody (Catalog # A09207). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

ATP2C2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-ATP2C2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09207)

Host

Rabbit

Contents

ATP2C2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

Clone: SK7

Isotype

IgG

Immunogen

ATP2C2 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human ATP2C2. The immunogen is located within the first 50 amino acids of ATP2C2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least three isoforms of ATP2C2 are known to exist. ATP2C2 antibody will not cross-react with ATP2C1

Reactive Species

A09207 is reactive to ATP2C2 in Human, Mouse

Applications

A09207 is guaranteed for ELISA, IF, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

103.169kDa

Background of ATP2C2

ATP2C2, also known as secretory pathway Ca2+/Mn2+-ATPase (SPCA) 2, belongs to the family of P-type cation transport ATPases. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium from the cytosol to the Golgi lumen. Defects in the related gene ATP2C1 cause Hailey-Hailey disease, for which ATP2C2 does not compensate, suggesting that ATP2C2 plays other physiological roles. Unlike ATP2C1, ATP2C2 has a much more restricted expression pattern and displays a higher maximal turnover rate for overall Ca2+-ATPase reaction and a lower apparent affinity for cytosolic Ca2+ activation of phosphorylation. Overexpression of ATP2C2 in mammary tumors result a Ca2+ influx via the store-operated Ca2+ channel ORAI1 and independent of the STIM1 and STIM2 sensors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ATP2C2 antibody can be used for detection of ATP2C2 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of ATP2C2 in 3T3 cell lysate with ATP2C2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.

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Immunofluorescence of ATP2C2 in 3T3 cells with ATP2C2 antibody at 20 μg/mL.

Gene/Protein Information For ATP2C2 (Source: Uniprot.org, NCBI)

Alternative Names

ATPase 2C2; ATPase, Ca++ transporting, type 2C, member 2; calcium-transporting ATPase type 2C member 2; DKFZp686H22230; KIAA0703; secretory pathway Ca(2+)-ATPase 2; secretory pathway calcium ATPase 2; SPCA2 ATP2C2 SPCA2 ATPase secretory pathway Ca2+ transporting 2 calcium-transporting ATPase type 2C member 2|ATPase 2C2|ATPase, Ca++ transporting, type 2C, member 2|secretory pathway Ca(2+)-ATPase 2|secretory pathway calcium ATPase 2

For more info on ATP2C2, check out the ATP2C2 Infographic

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In this infographic, you will see the following information for ATP2C2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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