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Product Info Summary
| SKU: | A32272 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-ATP5D Antibody (C-term)
SKU/Catalog Number
A32272
Size
80 µl
Description
Boster Bio Anti-ATP5D Antibody (C-term) (Catalog # A32272). Tested in WB application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ATP5D Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A32272)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This ATP5D antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 156-188 amino acids from the C-terminal region of human ATP5D.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A32272 is reactive to ATP5F1D in Human, Mouse, Rat
Applications
A32272 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
17.49kDa
Background of ATP5F1D
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP turnover in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000-1:4000
Validation Images & Assay Conditions
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Western blot analysis of lysates from A549, HepG2, Jurkat cell line, mouse brain tissue lysate, rat PC-12 cell line (from left to right), using ATP5D Antibody (C-term). A32272 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
Protein Target Info & Infographic
Gene/Protein Information For ATP5F1D (Source: Uniprot.org, NCBI)
Gene Name
ATP5F1D
Full Name
ATP synthase subunit delta, mitochondrial
Weight
17.49kDa
Superfamily
ATPase epsilon chain family
Alternative Names
ATP synthase subunit delta, mitochondrial ATP5F1D ATP5D, MC5DN5 ATP synthase F1 subunit delta ATP synthase subunit delta, mitochondrial|ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit|F-ATPase delta subunit|mitochondrial ATP synthase complex delta-subunit precusor|mitochondrial ATP synthase, delta subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATP5F1D, check out the ATP5F1D Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATP5F1D: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATP5D Antibody (C-term) (A32272)
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No publications found for A32272
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Customer Reviews
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