Anti-ATP5D Antibody (C-term)

Product Name

Anti-ATP5D Antibody (C-term)

View all ATP5F1D Antibodies

SKU/Catalog Number

A32272

Size

80 µl

Description

Boster Bio Anti-ATP5D Antibody (C-term) (Catalog # A32272). Tested in WB application(s). This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-ATP5D Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A32272)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This ATP5D antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 156-188 amino acids from the C-terminal region of human ATP5D.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A32272 is reactive to ATP5F1D in Human, Mouse, Rat

Reconstitution

Calculated molecular weight

17490 Da

Background of ATP5F1D

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP turnover in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A32272 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000-1:4000

Validation Images & Assay Conditions

Gene/Protein Information For ATP5F1D (Source: Uniprot.org, NCBI)

Alternative Names

ATP synthase subunit delta, mitochondrial, F-ATPase delta subunit, ATP5D ATP5F1D ATP5D, MC5DN5 ATP synthase F1 subunit delta ATP synthase subunit delta, mitochondrial|ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit|F-ATPase delta subunit|mitochondrial ATP synthase complex delta-subunit precusor|mitochondrial ATP synthase, delta subunit

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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