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Product Info Summary
|Application:||ELISA, IF, ICC|
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Anti-ATR Antibody Picoband™
Boster Bio Anti-ATR Antibody Picoband™ catalog # A00262-2. Tested in ELISA, IF, ICC applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-ATR Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00262-2)
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
E.coli-derived human ATR recombinant protein (Position: E24-D1324).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
A00262-2 is reactive to ATR in Human
A00262-2 is guaranteed for ELISA, IF, ICC Boster Guarantee
Background of ATR
Serine/threonine-protein kinase ATR also known as ataxia telangiectasia and Rad3-related protein (ATR) or FRAP-related protein 1 (FRP1) is an enzyme that, in humans, is encoded by the ATR gene. The protein encoded by this gene is a serine/threonine kinase and DNA damage sensor, activating cell cycle checkpoint signaling upon DNA stress. The encoded protein can phosphorylate and activate several proteins involved in the inhibition of DNA replication and mitosis, and can promote DNA repair, recombination, and apoptosis. This protein is also important for fragile site stability and centrosome duplication. Defects in this gene are a cause of Seckel syndrome 1.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. IF analysis of ATR using anti-ATR antibody (A00262-2).
ATR was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ATR Antibody (A00262-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Gene/Protein Information For ATR (Source: Uniprot.org, NCBI)
Serine/threonine-protein kinase ATR
ataxia telangiectasia and Rad3 related; Ataxia telangiectasia and Rad3-related protein; ATR; EC 188.8.131.52; FRAP-related protein 1; FRP1; FRP1FRAP-related protein-1; MEC1; protein kinase ATR; Rad3 related protein; SCKL1; SCKLMEC1, mitosis entry checkpoint 1, homolog; serine/threonine-protein kinase ATR ATR FCTCS, FRP1, MEC1, SCKL, SCKL1 ATR serine/threonine kinase serine/threonine-protein kinase ATR|FRAP-related protein-1|MEC1, mitosis entry checkpoint 1, homolog|ataxia telangiectasia and Rad3-related protein*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on ATR, check out the ATR Infographic
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In this infographic, you will see the following information for ATR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
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