Anti-ATRX Antibody Picoband™

ATRX antibody

Boster Bio Anti-ATRX Antibody Picoband™ catalog # A00203-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: A00203-2
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IHC, WB

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Product Name

Anti-ATRX Antibody Picoband™

View all ATRX Antibodies

SKU/Catalog Number

A00203-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ATRX Antibody Picoband™ catalog # A00203-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ATRX Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00203-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human ATRX recombinant protein (Position: E8-Q289).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00203-2 is reactive to ATRX in Human, Mouse, Rat

Applications

A00203-2 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

282 kDa

Calculated molecular weight

282.586kDa

Background of ATRX

Transcriptional regulator ATRX also known as ATP-dependent helicase ATRX, X-linked helicase II, or X-linked nuclear protein (XNP) is a protein that in humans is encoded by the ATRX gene. It is mapped to Xq21.1. The protein encoded by this gene contains an ATPase/helicase domain, and thus it belongs to the SWI/SNF family of chromatin remodeling proteins. This protein is found to undergo cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in the gene regulation at interphase and chromosomal segregation in mitosis. Mutations in this gene are associated with X-linked syndromes exhibiting cognitive disabilities as well as alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Flow Cytometry, 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For ATRX (Source: Uniprot.org, NCBI)

Gene Name

ATRX

Full Name

Transcriptional regulator ATRX

Weight

282.586kDa

Superfamily

SNF2/RAD54 helicase family

Alternative Names

alpha thalassemia/mental retardation syndrome X-linked (RAD54 (S. cerevisiae)homolog); alpha thalassemia/mental retardation syndrome X-linked (RAD54 homolog, S.cerevisiae); alpha thalassemia/mental retardation syndrome X-linked; ATP-dependent helicase ATRX; ATR2; DNA dependent ATPase and helicase; EC 3.6.1; EC 3.6.4.12; helicase 2, X-linked; Juberg-Marsidi syndrome; MGC2094; MRXHF1; RAD54 homolog; RAD54L; SFM1; SHS; transcriptional regulator ATRX; XH2RAD54; X-linked helicase II; X-linked nuclear protein; XNPZNF-HX; Zinc finger helicase; Znf-HX ATRX JMS, MRX52, RAD54, RAD54L, XH2, XNP, ZNF-HX ATRX chromatin remodeler transcriptional regulator ATRX|ATP-dependent helicase ATRX|X-linked helicase II|X-linked nuclear protein|alpha thalassemia/mental retardation syndrome X-linked (RAD54 homolog, S. cerevisiae)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ATRX, check out the ATRX Infographic

ATRX infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ATRX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A00203-2 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Co-occurrence of ATRX and IDH Mutations Identify Subgroup of Glioma Patients for Better Survival. Trivedi Trupti,Panchal Kinjal,Bhalala Neha,Jyotishi Charmi,Trivedi Priti,Panchal Harsha,Modi Nikhi
Species: Human
A00203-2 usage in article: APP:IHC, SAMPLE:GLIOMA TISSUE, DILUTION:1:100

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6 Customer Q&As for Anti-ATRX Antibody Picoband™

Question

Nuclear staining is expected and seen in the mouse/rat sections but the breast cancer tissue looks to have cytoplasmic staining and no nuclear staining.Would you happen to have additional IHC-P staining of human tissue for A00203-2?

Verified customer

Asked: 2020-10-01

Answer

Although nuclear staining on the breast cancer tissue was not obvious, weak nuclear staining could be observed. It is mainly cytoplasmic staining according to our in house test using the Anti-ATRX Antibody Picoband™ (A00203-2). Our lab hasn't got additional IHC-P staining of human tissues.

Boster Scientific Support

Answered: 2020-10-09

Question

We have been able to see staining in mouse liver. What should we do? Is anti-ATRX antibody supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2020-03-04

Answer

Based on literature liver does express ATRX. Based on Uniprot.org, ATRX is expressed in frontal cortex, brain, cervix carcinoma, embryonic kidney, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have ATRX expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15772651
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2020-03-04

Question

My question regards using your anti-ATRX antibody for positive regulation of telomeric rna transcription from rna pol ii promoter studies. Has this antibody been tested with western blotting on hela whole cell lysates? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-01-22

Answer

Thank you for your inquiry. This A00203-2 anti-ATRX antibody is tested on human hela, a549 cells, hela whole cell lysates. It is guaranteed to work for ELISA, Flow Cytometry, IHC-P, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-01-22

Question

My colleagues were happy with the WB result of your anti-ATRX antibody. However we have been able to see positive staining in brain pml using this antibody. Is that expected? Could you tell me where is ATRX supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-10-01

Answer

From literature, brain does express ATRX. Generally ATRX expresses in nucleus. chromosome, telomere. nucleus, pml. Regarding which tissues have ATRX expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15772651
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2018-10-01

Question

We are currently using anti-ATRX antibody A00203-2 for rat tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?

A. Gonzalez

Verified customer

Asked: 2017-10-26

Answer

The anti-ATRX antibody (A00203-2) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-10-26

Question

We ordered your anti-ATRX antibody for ELISA on leukemic t-cell a few months ago. I am using mouse, and I plan to use the antibody for WB next. My question regards examining leukemic t-cell as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

K. Taylor

Verified customer

Asked: 2014-02-04

Answer

I viewed the website and datasheets of our anti-ATRX antibody and it seems that A00203-2 has been validated on mouse in both ELISA and WB. Thus A00203-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2014-02-04

Order DetailsPrice
A00203-2

100μg

$370
A00203-2-10ug

10μg sample (liquid)

$99
A00203-2-Biotin

100 μg Biotin conjugated

$570
A00203-2-Cy3

100 μg Cy3 conjugated

$570
A00203-2-Dylight488

100 μg Dylight488 conjugated

$570
A00203-2-Dylight550

100 μg Dylight550 conjugated

$570
A00203-2-Dylight594

100 μg Dylight594 conjugated

$570
A00203-2-FITC

100 μg FITC conjugated

$570
A00203-2-HRP

100 μg HRP conjugated

$570
A00203-2-APC

100 μg APC conjugated

$670
A00203-2-PE

100 μg PE conjugated

$670
A00203-2-carrier-free

Carrier Free

$370

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A00203-2
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