Anti-Autophagy protein 5 ATG5 Antibody

Product Name

Anti-Autophagy protein 5 ATG5 Antibody

View all ATG5 Antibodies

SKU/Catalog Number

A00240

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Autophagy protein 5 ATG5 Antibody (Catalog # A00240). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

ATG5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Autophagy protein 5 ATG5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00240)

Host

Rabbit

Contents

ATG5 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

ATG5 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human ATG5. The immunogen is located within the first 50 amino acids of ATG5.

Cross-reactivity

Three isoforms of ATG5 are known to exist; this ATG5 antibody will only detect the longest isoform.

Reactive Species

A00240 is reactive to ATG5 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Background of ATG5

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG5, another member of the autophagy protein family, forms a conjugate with ATG12; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00240 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ATG5 antibody can be used for the detection of ATG5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information ATG5

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