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Product Info Summary
| SKU: | A08274 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Monkey |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Cell death regulator Aven Aven Antibody
SKU/Catalog Number
A08274
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Cell death regulator Aven Aven Antibody catalog # A08274. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cell death regulator Aven Aven Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08274)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human AVEN. AA range:301-350
Reactive Species
A08274 is reactive to AVEN in Human, Monkey
Observed Molecular Weight
39 kDa
Calculated molecular weight
38.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08274 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Aven Polyclonal Antibody
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Western blot analysis of lysates from COS7 and LOVO cells, using AVEN Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-Cell death regulator Aven Aven Antibody (A08274)
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3 Customer Q&As for Anti-Cell death regulator Aven Aven Antibody
Question
We are currently using anti-Aven antibody A08274 for monkey tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, monkey. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
The anti-Aven antibody (A08274) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-15
Question
Would anti-Aven antibody A08274 work for WB with cervix carcinoma?
K. Krishna
Verified customer
Asked: 2018-12-27
Answer
According to the expression profile of cervix carcinoma, AVEN is highly expressed in cervix carcinoma. So, it is likely that anti-Aven antibody A08274 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2018-12-27
Question
I was wanting to use your anti-Aven antibody for WB for human cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma identification?
B. Bhatt
Verified customer
Asked: 2014-12-16
Answer
You can see on the product datasheet, A08274 anti-Aven antibody has been validated for WB on human, monkey tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-12-16
