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Product Info Summary
| SKU: | A00986 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-Axin-1 AXIN1 Antibody
SKU/Catalog Number
A00986
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Axin-1 AXIN1 Antibody (Catalog # A00986). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
AXIN1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Axin-1 AXIN1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00986)
Host
Rabbit
Contents
AXIN1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
AXIN1 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human AXIN1. The immunogen is located within amino acids 620 - 670 of AXIN1.
Reactive Species
A00986 is reactive to AXIN1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
95.6 kDa
Background of AXIN1
AXIN1 is a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain and is thought to function as a negative regulator of the WNT signaling pathway that regulates embryonic axis formation. AXIN1 interacts with adenomatosis polyposis coli (APC), beta-catenin, glycogen synthase kinase 3 beta, forming a tetrameric complex resulting in the regulation of the stabilization of beta-catenin. Mutations in the AXIN1 gene have been associated various carcinomas, indicating that it also functions as a tumor suppressor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00986 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
AXIN1 antibody can be used for detection of AXIN1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of AXIN1 in SK-N-SH cell lysate with AXIN1 antibody at 1 μg/mL.
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Immunofluorescence of AXIN1 in human brain tissue with AXIN1 antibody at 20 μg/mL.
Specific Publications For Anti-Axin-1 AXIN1 Antibody (A00986)
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