Anti-Axin2/Conductin Rabbit Monoclonal Antibody

Axin-2 antibody

Boster Bio Anti-Axin2/Conductin Rabbit Monoclonal Antibody catalog # M01772. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M01772
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, ICC, WB

Product Name

Anti-Axin2/Conductin Rabbit Monoclonal Antibody

View all Axin-2 Antibodies

SKU/Catalog Number

M01772

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Axin2/Conductin Rabbit Monoclonal Antibody catalog # M01772. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Axin2/Conductin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01772)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AAIA-1

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Axin2

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01772 is reactive to AXIN2 in Human, Mouse, Rat

Applications

M01772 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

115-130 kDa

Calculated molecular weight

93.558kDa

Background of Axin-2

Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

Gene/Protein Information For AXIN2 (Source: Uniprot.org, NCBI)

Gene Name

AXIN2

Full Name

Axin-2

Weight

93.558kDa

Alternative Names

AXIL; axin 2; Axin2; Axin-2; Axin-like protein; Axis inhibition protein 2; Conductin; DKFZp781B0869; MGC10366; MGC126582 AXIN2 AXIL, ODCRCS axin 2 axin-2|axin-like protein|axis inhibition protein 2|conductin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on AXIN2, check out the AXIN2 Infographic

AXIN2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for AXIN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-Axin2/Conductin Rabbit Monoclonal Antibody

Question

I was wanting to use your anti-Axin2/Conductin Rabbit Monoclonal antibody for IF for human brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain identification?

Verified Customer

Verified customer

Asked: 2020-04-06

Answer

As indicated on the product datasheet, M01772 anti-Axin2/Conductin Rabbit Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-04-06

Question

Will anti-Axin2/Conductin Rabbit Monoclonal antibody M01772 work for IF with brain?

Verified Customer

Verified customer

Asked: 2020-03-25

Answer

According to the expression profile of brain, AXIN2 is highly expressed in brain. So, it is likely that anti-Axin2/Conductin Rabbit Monoclonal antibody M01772 will work for IF with brain.

Boster Scientific Support

Answered: 2020-03-25

Question

Does M01772 anti-Axin2/Conductin Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-02-17

Answer

You can see on the product datasheet, M01772 anti-Axin2/Conductin Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-02-17

Question

Can you help my question with product M01772, anti-Axin2/Conductin Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-07-05

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01772 anti-Axin2/Conductin Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-07-05

Question

We are currently using anti-Axin2/Conductin Rabbit Monoclonal antibody M01772 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2018-01-31

Answer

The anti-Axin2/Conductin Rabbit Monoclonal antibody (M01772) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-01-31

Order DetailsPrice
M01772

100μL

$370
M01772-30ul

30μL sample

$99
M01772-carrier-free

Carrier Free

$370

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Free Secondary Antibody

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M01772
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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