Product Info Summary
SKU: | M01772 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Axin2/Conductin Rabbit Monoclonal Antibody
SKU/Catalog Number
M01772
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Axin2/Conductin Rabbit Monoclonal Antibody catalog # M01772. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Axin2/Conductin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01772)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AAIA-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Axin2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01772 is reactive to AXIN2 in Human, Mouse, Rat
Applications
M01772 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
115-130 kDa
Calculated molecular weight
93.558kDa
Background of Axin-2
Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Axin2 expression in (1) SW480 cell lysate; (2) PC-12 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For AXIN2 (Source: Uniprot.org, NCBI)
Gene Name
AXIN2
Full Name
Axin-2
Weight
93.558kDa
Alternative Names
AXIL; axin 2; Axin2; Axin-2; Axin-like protein; Axis inhibition protein 2; Conductin; DKFZp781B0869; MGC10366; MGC126582 AXIN2 AXIL, ODCRCS axin 2 axin-2|axin-like protein|axis inhibition protein 2|conductin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AXIN2, check out the AXIN2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AXIN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Axin2/Conductin Rabbit Monoclonal Antibody (M01772)
Hello CJ!
No publications found for M01772
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Axin2/Conductin Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-Axin2/Conductin Rabbit Monoclonal Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-Axin2/Conductin Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-Axin2/Conductin Rabbit Monoclonal antibody for IF for human brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain identification?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
As indicated on the product datasheet, M01772 anti-Axin2/Conductin Rabbit Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-06
Question
Will anti-Axin2/Conductin Rabbit Monoclonal antibody M01772 work for IF with brain?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
According to the expression profile of brain, AXIN2 is highly expressed in brain. So, it is likely that anti-Axin2/Conductin Rabbit Monoclonal antibody M01772 will work for IF with brain.
Boster Scientific Support
Answered: 2020-03-25
Question
Does M01772 anti-Axin2/Conductin Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
You can see on the product datasheet, M01772 anti-Axin2/Conductin Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-17
Question
Can you help my question with product M01772, anti-Axin2/Conductin Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-07-05
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01772 anti-Axin2/Conductin Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-07-05
Question
We are currently using anti-Axin2/Conductin Rabbit Monoclonal antibody M01772 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-01-31
Answer
The anti-Axin2/Conductin Rabbit Monoclonal antibody (M01772) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-31