Product Info Summary
SKU: | M00226 |
---|---|
Size: | 80 µl |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-AXL Antibody (C-term)
SKU/Catalog Number
M00226
Size
80 µl
Description
Boster Bio Anti-AXL Antibody (C-term) (Catalog # M00226). Tested in WB application(s). This antibody reacts with Human, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-AXL Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00226)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This AXL antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 838-872 amino acids from the C-terminal region of human AXL.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00226 is reactive to AXL in Human, Rat
Applications
M00226 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
98.337kDa
Background of AXL
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, ALX binds and induces tyrosine phosphorylation of PI3- kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TENC1. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response. In case of filovirus infection, seems to function as a cell entry factor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
Validation Images & Assay Conditions
Click image to see more details
All lanes : Anti-AXL Antibody (C-term) at 1:2000 dilution
Lane 1: NCI-H1299 whole cell lysates
Lane 2: Hela whole cell lysates
Lane 3: L6 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 98 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Protein Target Info & Infographic
Gene/Protein Information For AXL (Source: Uniprot.org, NCBI)
Gene Name
AXL
Full Name
Tyrosine-protein kinase receptor UFO
Weight
98.337kDa
Superfamily
protein kinase superfamily
Alternative Names
AI323647; ARK; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming sequence/gene; Axl; EC 2.7.10; EC 2.7.10.1; JTK11; Tyro7; tyrosine-protein kinase receptor UFO; UFO; UFOoncogene AXL AXL ARK, JTK11, Tyro7, UFO AXL receptor tyrosine kinase tyrosine-protein kinase receptor UFO|AXL oncogene|AXL transforming sequence/gene
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AXL, check out the AXL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AXL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AXL Antibody (C-term) (M00226)
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No publications found for M00226
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Customer Reviews
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