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- Table of Contents
AXL antibody (M00226-7) performs well in Flow Cytometry, IF, ICC, WB and shows reactivity with Human, Monkey.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Western blot analysis of AXL using anti-AXL antibody (M00226-7).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AXL antigen affinity purified monoclonal antibody (M00226-7) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for AXL at approximately 140 kDa. The expected band size for AXL is at 98 kDa.
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AXL antigen affinity purified monoclonal antibody (M00226-7) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for AXL at approximately 140 kDa. The expected band size for AXL is at 98 kDa.
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Anti-AXL Antibody (Monoclonal, 34A02)
AXL antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Monkey | Rabbit | Flow Cytometry, IF, ICC, WB |
Tested Applications
AXL antibody was positively detected by WB in:
human U-87MG whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
1:500-2000
Immunocytochemistry/Immunofluorescence
1:50-200
Flow Cytometry (Fixed)
1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
ICC
IF
FC
Human
Tested
Recommend
Recommend
Recommend
Monkey
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-AXL Antibody (Monoclonal, 34A02)
View all AXL Antibodies
View all AXL Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
34A02
Immunogen
Recombinant protein within human Axl aa 3-449.
Observed Molecular Weight
140 kDa
Calculated Molecular Weight
98.3 kDa
Background of AXL
Tyrosine-protein kinase receptor UFO is an enzyme that in humans is encoded by the AXL gene. This gene is mapped to 19q13.2. The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal,followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth,migration,aggregation and anti-inflammation in multiple cell types. Alternative splicing results in multiple transcript variants of this gene.
Cite This Product
Anti-AXL Antibody (Monoclonal, 34A02) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00226-7)
Protocols
Western Blot protocol for AXL antibody
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Product Promise
We promise Anti-AXL Antibody (Monoclonal, 34A02) performs excellently in relevant applications for Flow Cytometry, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M00226-7
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026