Product Info Summary
SKU: | A02597 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-B MyB/MYBL2 Antibody Picoband™
SKU/Catalog Number
A02597
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-B MyB/MYBL2 Antibody Picoband™ catalog # A02597. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-B MyB/MYBL2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02597)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human B MyB/MYBL2 recombinant protein (Position: K31-R695).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02597 is reactive to MYBL2 in Human
Applications
A02597 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
100 kDa
Calculated molecular weight
78.764kDa
Background of B-Myb
MYBL2 (V-MYB avian myeloblastosis viral oncogene homolog-like 2), also called MYB-RELATED GENE BMYB, is a protein that in humans is encoded by the MYBL2 gene. The protein encoded by this gene, a member of the MYB family of transcription factor genes, is a nuclear protein involved in cell cycle progression. Barletta et al. (1991) assigned the MYBL2 gene to chromosome Xq13. However, Noben-Trauth et al. (1996) demonstrated that this assignment was an error. Using mouse Mybl2 cDNA clones as probes, they assigned Mybl2 in an interspecific backcross panel to distal mouse chromosome 2. Using human cDNA probes in combination with fluorescence in situ hybridization analysis, they localized MYBL2 to chromosome 20q13.1, a region that is commonly deleted in myeloid disorders and shows high homology of synteny to mouse chromosome 2. It has been shown to activate the cell division cycle 2, cyclin D1, and insulin-like growth factor-binding protein 5 genes. Transcript variants may exist for this gene, but their full-length natures have not been determined.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of B MyB/MYBL2 using anti-B MyB/MYBL2 antibody (A02597).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human HEK293 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-B MyB/MYBL2 antigen affinity purified polyclonal antibody (Catalog # A02597) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for B MyB/MYBL2 at approximately 100KD. The expected band size for B MyB/MYBL2 is at 100KD.
Click image to see more details
Figure 2. IF analysis of B MyB/MYBL2 using anti-B MyB/MYBL2 antibody (A02597).
B MyB/MYBL2 was detected in immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-B MyB/MYBL2 Antibody (A02597) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 3. Flow Cytometry analysis of U937 cells using anti-B MyB/MYBL2 antibody (A02597).
Overlay histogram showing U937 cells stained with A02597 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-B MyB/MYBL2 Antibody (A02597, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For MYBL2 (Source: Uniprot.org, NCBI)
Gene Name
MYBL2
Full Name
Myb-related protein B
Weight
78.764kDa
Alternative Names
B-MYB; BMYBMGC15600; Myb-like protein 2; myb-related protein B; v-myb avian myeloblastosis viral oncogene homolog-like 2; v-myb myeloblastosis viral oncogene homolog (avian)-like 2 MYBL2 B-MYB, BMYB MYB proto-oncogene like 2 myb-related protein B|myb-like protein 2|v-myb avian myeloblastosis viral oncogene homolog-like 2|v-myb myeloblastosis viral oncogene homolog-like 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MYBL2, check out the MYBL2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MYBL2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-B MyB/MYBL2 Antibody Picoband™ (A02597)
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No publications found for A02597
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